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Invitrogen

CD254 (RANK Ligand) Monoclonal Antibody (MIH24), Biotin, eBioscience™

2 Published Figures
2 References
View all (77) RANKL (RANK Ligand) antibodies

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Product Details

13-6619-82

Applications
Tested Dilution
Publications

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-Dependent
View 1 publication 1 publication

ELISA (ELISA)

Assay-Dependent
-
Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2b, kappa

Recommended Isotype Control

Mouse IgG2b kappa Isotype Control (eBMG2b), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MIH24

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Amount

100 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466887

Product Specific Information

Description: The MIH24 monoclonal antibody reacts with human TRANCE (TNF-related activation-induced cytokine receptor) also known as RANKL (receptor activator of NF-kappaB ligand) and OPGL (Osteoprotegerin Ligand). TRANCE is a type II membrane protein with predicted molecular weight of 35 kDa. Its extracellular domain is most closely related to TRAIL, FasL and TNF. TRANCE mRNA is upregulated by TCR stimulation. TRANCE-deficient mouse lacks osteoclasts. In addition, TRANCE has been shown to be involved in the interactions between T-dendritic and T-B cells. It was also found to be critical in osteoclast differentiation. The TRANCE-RANK interaction regulates the expression of the anti-apoptotic molecule Bcl-xL.

Applications Reported: The MIH24 antibody has been reported for use in flow cytometric analysis, and ELISA.

Applications Tested: The MIH24 antibody has been tested by ELISA. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CD254; H-RANK-L; M-Rank-L; ODF; OPGL; Osteoclast differentiation factor; Osteoprotegerin ligand; rank-l; RANKL; receptor activator of nuclear factor kappa B ligand; Receptor activator of nuclear factor kappa-B ligand; RP11-86N24.2; srankl; TNF-related activation-induced cytokine; TNF-related ligand; TRANCE; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand 6B; Tumor necrosis factor ligand superfamily member 11; tumor necrosis factor superfamily member 11

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Gene Aliases: CD254; hRANKL2; ODF; OPGL; OPTB2; RANKL; sOdf; TNFSF11; TNLG6B; TRANCE

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UniProt ID: (Human) O14788

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Entrez Gene ID: (Human) 8600

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Function(s)
receptor binding cytokine activity tumor necrosis factor receptor binding protein binding tumor necrosis factor receptor superfamily binding identical protein binding receptor agonist activity
Process(es)
negative regulation of transcription from RNA polymerase II promoter ossification osteoclast proliferation monocyte chemotaxis immune response cell communication cell surface receptor signaling pathway JNK cascade organ morphogenesis positive regulation of gene expression cytokine-mediated signaling pathway calcium-mediated signaling signaling cell differentiation osteoclast differentiation tumor necrosis factor-mediated signaling pathway mammary gland epithelial cell proliferation positive regulation of homotypic cell-cell adhesion osteoclast development paracrine signaling positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of MAPK cascade protein kinase B signaling tooth eruption bone resorption regulation of osteoclast differentiation positive regulation of osteoclast differentiation positive regulation of bone resorption positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade positive regulation of T cell activation positive regulation of corticotropin-releasing hormone secretion positive regulation of protein kinase B signaling calcium ion homeostasis bone development mammary gland alveolus development positive regulation of ERK1 and ERK2 cascade positive regulation of fever generation by positive regulation of prostaglandin secretion positive regulation of NIK/NF-kappaB signaling positive regulation of intracellular signal transduction cellular response to leukemia inhibitory factor positive regulation of osteoclast development positive regulation of extrinsic apoptotic signaling pathway
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