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Invitrogen

CD284 (TLR4) Monoclonal Antibody (HTA125), Biotin, eBioscience™

34 Published Figures
34 References
View all (74) TLR4 (CD284) antibodies

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Product Details

13-9917-82

Applications
Tested Dilution
Publications

Western Blot (WB)

-
View 2 publications 2 publications

Immunohistochemistry (IHC)

-
View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

-
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-
View 2 publications 2 publications

Flow Cytometry (Flow)

1 µg/test
View 16 publications 16 publications

ELISA (ELISA)

-
View 1 publication 1 publication

Neutralization (Neu)

-
View 8 publications 8 publications

Functional Assay (Functional)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human

Published species

Human, Shark

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

HTA125

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466984

Product Specific Information

Description: The HTA125 monoclonal antibody reacts with human Toll-like receptor 4 (TLR4). So far, at least ten members of the Toll family have been identified in humans. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. TLR4 physically associates with another molecule called MD-2, and together with CD14, this complex is responsible for LPS recognition and signaling. TLR4 is expressed by peripheral blood monocytes. HTA125 has been reported to immunoprecipitate human TLR4 (~100 kDa) from transfected cells. Most TLR cell surface expression, especially TLR1 and TLR4, occurs at low levels on monocytes and at even lower levels on other cell types including granulocytes and immature dendritic cells (iDC). Furthermore, a relatively high degree of variability in TLR surface expression has been reported among normal donors.

It is highly recommended that for optimal staining of TLR4, whole blood be stained using the lysed whole blood protocol (found in BestProtocols®) rather than Ficoll-gradient prepared normal human peripheral blood cells. The use of a density gradient appears to reduce the staining intensity significantly.

Applications Reported:The HTA125 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The HTA125 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TLR4 is member of the Toll like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. This receptor is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. Mammalian cells respond to LPS by activating TLR4. TLR4 belongs to the multi-protein complex of lipopolysaccharide (LPS) receptor, containing CD14, LY96 and TLR4, and is involved in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. TLR4 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. Mutations in the TLR4 gene have been associated with differences in LPS responsiveness. Also, several transcript variants of the TLR4 gene have been found, but the protein coding potential of most of them is uncertain. TLR4 is expressed by peripheral blood monocytes and a small population of B-cells and is also expressed in human placenta. Studies with TLR4-deficient mice indicate that the main ligand for TLR is lipopolysaccharide. Consequently, these mice also showed increased susceptibility to Gram-negative sepsis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CD284; homolog of Drosophila toll; hToll; similar to the Drosophila Toll protein; LRR motif; similar to the cytoplasmic domain of IL-1 receptor; toll like receptor 4 protein; Toll-like receptor 4; unnamed protein product

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Gene Aliases: ARMD10; CD284; TLR-4; TLR4; TOLL

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UniProt ID: (Human) O00206

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Entrez Gene ID: (Human) 7099

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Function(s)
lipopolysaccharide binding beta-amyloid binding lipopolysaccharide receptor activity transmembrane signaling receptor activity receptor binding protein binding signaling receptor activity identical protein binding protein heterodimerization activity NAD+ nucleotidase, cyclic ADP-ribose generating
Process(es)
activation of innate immune response toll-like receptor signaling pathway wound healing involved in inflammatory response B cell proliferation involved in immune response immune system process nitric oxide production involved in inflammatory response regulation of dendritic cell cytokine production MyD88-dependent toll-like receptor signaling pathway nitric oxide biosynthetic process phagocytosis defense response inflammatory response immune response signal transduction I-kappaB kinase/NF-kappaB signaling JNK cascade response to bacterium gene expression positive regulation of platelet activation positive regulation of gene expression astrocyte development microglia differentiation positive regulation of smooth muscle cell migration detection of fungus neurogenesis signal transduction involved in regulation of gene expression positive regulation of B cell proliferation lipopolysaccharide-mediated signaling pathway response to lipopolysaccharide detection of lipopolysaccharide regulation of tumor necrosis factor production negative regulation of interferon-gamma production negative regulation of interleukin-17 production negative regulation of interleukin-23 production negative regulation of interleukin-6 production negative regulation of tumor necrosis factor production positive regulation of chemokine production positive regulation of interferon-alpha production positive regulation of interferon-beta production positive regulation of interferon-gamma production positive regulation of interleukin-1 beta production positive regulation of interleukin-1 production positive regulation of interleukin-10 production positive regulation of interleukin-12 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of tumor necrosis factor production positive regulation of stress-activated MAPK cascade toll-like receptor 4 signaling pathway regulation of toll-like receptor 4 signaling pathway TRIF-dependent toll-like receptor signaling pathway cellular response to platelet-derived growth factor stimulus T-helper 1 type immune response B cell proliferation macrophage activation defense response to bacterium positive regulation of macrophage activation positive regulation of I-kappaB kinase/NF-kappaB signaling innate immune response leukocyte activation MHC class II biosynthetic process positive regulation of MHC class II biosynthetic process positive regulation of nitric oxide biosynthetic process negative regulation of osteoclast differentiation positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade lymphocyte proliferation positive regulation of smooth muscle cell proliferation positive regulation of lymphocyte proliferation regulation of inflammatory response positive regulation of inflammatory response defense response to Gram-negative bacterium positive regulation of NF-kappaB transcription factor activity NK T cell activation stress-activated MAPK cascade detection of stimulus intestinal epithelial structure maintenance positive regulation of macrophage cytokine production ERK1 and ERK2 cascade negative regulation of ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade nucleotide-binding oligomerization domain containing 1 signaling pathway positive regulation of nucleotide-binding oligomerization domain containing 1 signaling pathway nucleotide-binding oligomerization domain containing 2 signaling pathway positive regulation of nucleotide-binding oligomerization domain containing 2 signaling pathway cellular response to lipopolysaccharide cellular response to lipoteichoic acid cellular response to mechanical stimulus cellular response to interferon-gamma negative regulation of cold-induced thermogenesis cellular response to oxidised low-density lipoprotein particle stimulus negative regulation of cytokine production involved in inflammatory response positive regulation of cytokine production involved in inflammatory response positive regulation of NLRP3 inflammasome complex assembly positive regulation of reactive oxygen species biosynthetic process positive regulation of cellular response to macrophage colony-stimulating factor stimulus positive regulation of matrix metallopeptidase secretion cellular response to beta-amyloid positive regulation of chemokine (C-X-C motif) ligand 2 production positive regulation of extrinsic apoptotic signaling pathway
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