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Invitrogen
CD13 Monoclonal Antibody (WM-15 (WM15)), eBioscience™
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FIGURE: 1 / 6
CD13 Antibody (14-0138-82) in Flow
Staining of normal human peripheral blood cells with 0.5 µg of Mouse IgG1 Isotype Control Purified (Product # 14-4714-82) (open histogram) or 0.5 µg of Anti-Human CD13 Purified (filled histogram) followed by Anti-Mouse IgG FITC (Product # 11-4011-85). Cells in the monocyte (left) and granulocyte (right) gates were used for analysis.
Product Details
14-0138-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
WM-15 (WM15)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The WM-15 monoclonal antibody reacts with human CD13, also known as aminopeptidase N. CD13 is a transmembrane ectoenzyme occurring on monocytes and granulocytes but not lymphocytes in blood. It is also reportedly on a wide variety of cells and in a wide variety of tissues, including endothelial cells, epithelial cells, fibroblasts, kidney proximal tubules, intestine, and placenta. CD13 is reported to have many different functions including degradation of enkephalins and endorphins, terminal degradation of peptides, amino acid scavenging, antigen processing, and adhesion and migration of cells.
Applications Reported: The WM-15 (WM15) antibody) has been reported for use in flow cytometric analysis, and microscopy.
Applications Tested: The WM-15 (WM15) antibody has been tested by flow cytometric analysis of lysed whole blood. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD13, also known as ANPEP, is a 150-170 kDa type II transmembrane zinc-binding ectopeptidase expressed on a wide variety of cell types and tissues. It is prominently found on monocytes and granulocytes in the blood, but not on lymphocytes. Additionally, CD13 is expressed on endothelial cells, epithelial cells, fibroblasts, kidney proximal tubules, intestine, placenta, and myeloid leukemia cells. As a metalloprotease, CD13 preferentially catalyzes the removal of neutral amino acids from small peptides, thereby activating or inactivating bioactive peptides. It plays a crucial role in various biological processes, including the degradation of enkephalins and endorphins, terminal degradation of peptides, amino acid scavenging, antigen processing, and the adhesion and migration of cells. CD13 is also involved in extracellular matrix degradation, signal transduction, inflammatory responses, regulation of intercellular contact, cell motility, and vascularization. In the context of disease, CD13 is associated with the protection of leukemic cells against apoptosis and is linked to a poor prognosis in carcinomas. Furthermore, CD13 functions as an aminopeptidase enzyme and serves as an alpha receptor for coronavirus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: alanyl (membrane) aminopeptidase; Alanyl aminopeptidase; Aminopeptidase M; Aminopeptidase N; aminopeptidase N precursor (EC 3.4.11.2); AP-M; AP-N; CD13; gp150; h AP-N; membrane alanyl aminopeptidase; Microsomal aminopeptidase; Myeloid plasma membrane glycoprotein CD13; unnamed protein product
Gene Aliases: ANPEP; AP-M; AP-N; APN; CD13; GP150; hAPN; LAP1; P150; PEPN
UniProt ID: (Human) P15144
Entrez Gene ID: (Human) 290
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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