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Invitrogen
CD40 Monoclonal Antibody (5C3), eBioscience™
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FIGURE: 1 / 9
CD40 Antibody (14-0409-82) in Flow
Reproductive biology and endocrinology : RB&E 2021 -
Fig. 2 rhIL-37 increased the serum Th1/Th2 ratio and promoted DCs to mature in the endometriosis mouse model. At 24 h after the last rhIL-37 administration, ( A - C ) the percentages of Th1 and Th2 cells, and the ratio of Th1/Th2 cells in serum were analyzed using flow cytometry; ( D - G ) the percentages of CD40-, CD80-, CD86-, and MHC II-positive DCs in serum were determined using flow cytometry. N = 6. *... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
14-0409-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
5C3
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The 5C3 monoclonal antibody reacts with human CD40, a 45-50 kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. CD40 regulates B cell development and maturation by inducing Ig isotype-switching and in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with CD154 (gp39), its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation. 5C3 is reported to be used for activation of B cells in in vitro functional assays.
Applications Reported: The 5C3 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections. 5C3 has also been reported in in vitro functional studies. (Please use Functional Grade purified 5C3, Product # 16-0409, in functional assays).
Applications Tested: The 5C3 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD40 is a single-chain glycoprotein and a member of the tumor necrosis factor receptor (TNFR) family, exhibiting significant homology to the Hodgkin's disease-associated antigen, CD30. It is expressed by B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells, as well as some epithelial cells, carcinomas, and lymphoid dendritic cells. Notably, CD40 is present on all B cells except plasma cells. CD40 plays a crucial role in regulating B cell development and maturation, inducing immunoglobulin isotype-switching, and protecting B cells from surface Ig-induced apoptosis when combined with other signals such as IL-4. It promotes proliferation and is essential for T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. The interaction between CD40 and its ligand CD154 (gp39) on T cells is vital for T-B cell crosstalk, costimulation, and immune regulation. Adaptor protein TNFR2 interacts with CD40, mediating signal transduction, while the AT-hook transcription factor AKNA is reported to regulate CD40 expression, which may be important for homotypic cell interactions. Additionally, the interaction between CD40 and its ligand is necessary for amyloid-beta-induced microglial activation, suggesting a role in the early pathogenesis of Alzheimer's disease. Two alternatively spliced transcript variants encoding distinct isoforms of CD40 have been identified. Diseases associated with CD40 dysfunction include Type 3 Hyper-IgM immunodeficiency and CD40 ligand deficiency, highlighting its importance in immune and inflammatory responses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: B cell surface antigen CD40; B cell-associated molecule; CD antigen CD40; CD40 molecule, TNF receptor superfamily member 5; CD40L receptor; Immunoglobulin M; ImmunoglobulinM; MGC9013; sCD40; soluble CD40
Gene Aliases: Bp50; CDW40; p50; TNFRSF5
UniProt ID: (Human) P25942
Entrez Gene ID: (Human) 958
immune response-regulating cell surface receptor signaling pathway
cellular calcium ion homeostasis
defense response
inflammatory response
JAK-STAT cascade
regulation of gene expression
B cell mediated immunity
CD40 signaling pathway
platelet activation
B cell proliferation
B cell activation
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of MAPK cascade
positive regulation of blood vessel endothelial cell migration
positive regulation of angiogenesis
positive regulation of transcription from RNA polymerase II promoter
regulation of immune response
positive regulation of developmental process
positive regulation of multicellular organismal process
protein-containing complex assembly
cellular response to mechanical stimulus
positive regulation of protein kinase C signaling
positive regulation of interleukin-4-mediated signaling pathway
positive regulation of endothelial cell apoptotic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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