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Invitrogen
CD45.1 Monoclonal Antibody (A20), eBioscience™
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FIGURE: 1 / 28
CD45.1 Antibody (14-0453-82) in Flow
Staining of SJL splenocytes with 0.25 µg of Mouse IgG2a K Isotype Control Purified (Product # 14-4724-82) (open histogram) or 0.25 µg of Anti-Mouse CD45.1 Purified (filled histogram) followed by Anti-Mouse IgG FITC (Product # 11-4011-85). Cells in the lymphocyte gate were used for analysis.
Product Details
14-0453-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
C. elegans,
Fish,
Human,
Mouse
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
A20
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The A20 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.1-expressing mouse strains. The strains that express CD45.1 include SJL/J, DA, STS/A and RIII. CD45.1 is expressed by all leukocytes in these strains.
Applications Reported: The A20 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections. (Biotinylated A20 is recommended for IHC, Product # 13-0453).
Applications Tested: The A20 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD45; CD45 antigen; GP180; L-CA; Lca; Leukocyte common antigen; Ly-5; LY5; Lymphocyte antigen 5; lymphocyte common antigen; Receptor-type tyrosine-protein phosphatase C; RT7; T200; T200 glycoprotein
Gene Aliases: B220; Cd45; CD45R; L-CA; loc; Ly-5; Lyt-4; Ptprc; T200
UniProt ID: (Mouse) P06800
Entrez Gene ID: (Mouse) 19264
activation of MAPK activity
negative regulation of T cell mediated cytotoxicity
positive regulation of T cell mediated cytotoxicity
negative regulation of cytokine-mediated signaling pathway
hematopoietic progenitor cell differentiation
immunoglobulin biosynthetic process
positive regulation of T cell mediated immunity
regulation of humoral immune response mediated by circulating immunoglobulin
positive regulation of humoral immune response mediated by circulating immunoglobulin
negative regulation of protein kinase activity
protein dephosphorylation
negative regulation of cell adhesion involved in substrate-bound cell migration
leukocyte cell-cell adhesion
response to gamma radiation
dephosphorylation
B cell differentiation
T cell differentiation
positive regulation of B cell proliferation
negative regulation of protein autophosphorylation
heterotypic cell-cell adhesion
peptidyl-tyrosine dephosphorylation
T cell proliferation
B cell proliferation
positive regulation of T cell proliferation
positive regulation of MAPK cascade
cell cycle phase transition
positive thymic T cell selection
negative thymic T cell selection
regulation of B cell differentiation
positive regulation of T cell differentiation
positive regulation of gamma-delta T cell differentiation
positive regulation of alpha-beta T cell proliferation
positive regulation of isotype switching to IgG isotypes
bone marrow development
stem cell development
negative regulation of peptidyl-tyrosine phosphorylation
T cell receptor signaling pathway
B cell receptor signaling pathway
regulation of B cell receptor signaling pathway
positive regulation of antigen receptor-mediated signaling pathway
release of sequestered calcium ion into cytosol
defense response to virus
regulation of cell cycle
positive regulation of hematopoietic stem cell migration
positive regulation of stem cell proliferation
regulation of extrinsic apoptotic signaling pathway
positive regulation of extrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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