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Invitrogen

CD61 (Integrin beta 3) Monoclonal Antibody (2C9.G3), eBioscience™

19 Published Figures
19 References
View all (103) CD61 (Integrin beta 3) antibodies

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Product Details

14-0611-85

Applications
Tested Dilution
Publications

Western Blot (WB)

-
View 2 publications 2 publications

Immunohistochemistry (IHC)

-
View 3 publications 3 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent
-

Immunocytochemistry (ICC/IF)

-
View 3 publications 3 publications

Flow Cytometry (Flow)

0.5 µg/test
View 9 publications 9 publications

Neutralization (Neu)

Assay-Dependent
View 2 publications 2 publications

Functional Assay (Functional)

Assay-Dependent
-
Product Specifications

Species Reactivity

Mouse, Rat

Published species

Mouse

Host/Isotype

Armenian hamster / IgG

Class

Monoclonal

Type

Antibody

Clone

2C9.G3

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Amount

500 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_467312

Product Specific Information

Description: The 2C9.G3 (HMb3-1) monoclonal antibody reacts with mouse and rat CD61, also known as the integrin beta3. CD61 is expressed by activated T cells, granulocytes, and platelet. CD61 associates non-covalently with two integrin alpha subunits; alphaV (CD51) to form Vitronectin Receptor and alphaIib (CD41) to form gpIIb/IIIa. These heterodimeric complexes are responsible for adhesion to extracellular matrix components including fibrinogen, fibronectin, fibronectin, vitronectin, thrombospondin and von Willebrand factor.

Applications Reported: The 2C9.G3 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections. 2C9.G3 has also been reported in blocking of ligand binding and some adhesive processes. (Please use Functional Grade purified 2C9.G3, Product # 16-0611, in functional assays).

Applications Tested: The 2C9.G3 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD61, also known as GPIIIa or ITGB3, is a 105 kDa glycoprotein expressed on activated T cells, granulocytes, megakaryocytes, platelets, and their precursors. It plays a crucial role in platelet aggregation and functions as a receptor for fibrinogen, fibronectin, von Willebrand factor, vitronectin, and thrombospondin. CD61 forms heterodimeric complexes by associating non-covalently with integrin alpha subunits: alphaV (CD51) to create the Vitronectin Receptor and alphaIIb (CD41) to form gpIIb/IIIa. These complexes are responsible for adhesion to extracellular matrix components, facilitating cell adhesion and cell-surface mediated signaling. CD61 is expressed on platelets and megakaryocytes in association with CD41, and on endothelial cells, monocytes, and osteoclasts in association with CD51. The protein product of CD61 is composed of an alpha chain and a beta chain, which can combine with multiple partners to form different integrins. Its involvement in cell adhesion and signaling underscores its importance in normal physiological processes. Dysfunction of CD61 is associated with diseases such as Glanzmann Thrombasthenia and Platelet type-16 Bleeding Disorder, highlighting its critical role in hemostasis and platelet function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CD61; GPIIIa; integrin beta 3 (Cd61); Integrin beta-3; platelet gpIIIa; Platelet membrane glycoprotein IIIa

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Gene Aliases: CD61; GP3A; INGRB3; Itgb3

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UniProt ID: (Rat) Q8R2H2, (Mouse) O54890

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Entrez Gene ID: (Rat) 29302, (Mouse) 16416

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Function(s)
fibronectin binding protease binding protein disulfide isomerase activity integrin binding protein binding peptide binding identical protein binding vascular endothelial growth factor receptor 2 binding cell adhesion molecule binding extracellular matrix binding fibrinogen binding receptor activity integrin
Process(es)
positive regulation of endothelial cell proliferation positive regulation of leukocyte migration protein folding cellular calcium ion homeostasis cell-substrate junction assembly cell adhesion cell-matrix adhesion integrin-mediated signaling pathway embryo implantation regulation of G-protein coupled receptor protein signaling pathway response to radiation positive regulation of endothelial cell migration negative regulation of macrophage derived foam cell differentiation positive regulation of fibroblast migration negative regulation of lipid storage response to activity smooth muscle cell migration positive regulation of smooth muscle cell migration cell growth cell migration platelet activation regulation of cell migration positive regulation of cell migration cell-substrate adhesion activation of protein kinase activity negative regulation of lipid transport positive regulation of cell adhesion mediated by integrin positive regulation of osteoblast proliferation heterotypic cell-cell adhesion substrate adhesion-dependent cell spreading cellular response to drug response to platelet-derived growth factor apolipoprotein A-I-mediated signaling pathway positive regulation of MAPK cascade negative regulation of low-density lipoprotein particle receptor biosynthetic process positive regulation of angiogenesis positive regulation of bone resorption viral entry into host cell mesodermal cell differentiation positive regulation of smooth muscle cell proliferation positive regulation of peptidyl-tyrosine phosphorylation negative regulation of lipoprotein metabolic process negative chemotaxis regulation of release of sequestered calcium ion into cytosol negative regulation of cell death positive regulation of ERK1 and ERK2 cascade platelet aggregation cellular response to mechanical stimulus positive regulation of glomerular mesangial cell proliferation positive regulation of adenylate cyclase-inhibiting opioid receptor signaling pathway cellular response to insulin-like growth factor stimulus positive regulation of osteoclast differentiation cell projection morphogenesis angiogenesis involved in wound healing
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