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Invitrogen
CD79a Monoclonal Antibody (24C2.5), eBioscience™
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FIGURE: 1 / 2
CD79a Antibody (14-0791-82) in WB
Western blot was performed using Anti-CD79a Monoclonal Antibody (24C2.5), eBioscience™ (Product # 14-0791-82) and a 40kDa band corresponding to CD79a was observed across cell lines tested. Whole cell extracts (30 µg lysate) of Raji (Lane 1), Ramos (Lane 2), Daudi (Lane 3), HL-60 (Lane 4), SH-SY5Y (Lane 5), NTERA-2 cl.D1 (Lane 6) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0342BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product... View More
Product Details
14-0791-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
24C2.5
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This 24C2.5 monoclonal antibody reacts with the intracellular tail of mouse CD79a (also known as Ig alpha or mb-1), a signaling component of the B cell receptor (BCR). CD79a heterodimerizes with CD79b (Ig beta, B29), and together with surface Ig, make up the BCR. Both CD79 subunits consist of a single extracellular Ig domain, a transmembrane domain, and an intracellular signaling domain. CD79a is expressed almost exclusively on B cells, as well as in most B lineage acute lymphoblastic leukemias. The cytoplasmic domain of CD79a contains immunoreceptor tyrosine-based activation motifs (ITAMs), which constitute the signal transducing portions of the BCR. Tyrosine phosphorylation of these ITAMs by Syk and Lyn initiates numerous signaling cascades, resulting in B cell activation, proliferation, and differentiation.
Applications Reported: This 24C2.5 antibody has been reported for use in immunoblotting (WB).
Applications Tested: This 24C2.5 antibody has been tested by western blot of reduced mouse spleen lysates. This can be used at 1-10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD79a is a disulphide-linked heterodimer that includes B29 (CD79b) polypeptide. CD79a is a B lymphocyte antigen receptor with an antigen-specific surface component Ig (immunoglobulin) that associates with Ig-alpha and Ig-beta, necessary elements for the expression and function of the B-cell antigen receptor. CD79a first appears at pre-B cell stage and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in the majority of acute leukemias of precursor B cell type, in B cell lines, B cell lymphomas, and in some myelomas. It is not present in myeloid or T cell lines. Diseases associated with CD79a include Agammaglobulinemia 3 and Agammaglobulinemia, Non-Bruton type.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: B-cell antigen receptor complex-associated protein alpha chain; CD79; CD79a; CD79a antigen (immunoglobulin-associated alpha); CD79a molecule, immunoglobulin-associated alpha; extracellular domain; Ig alpha; Ig-alpha; immunoglobulin-associated alpha; MB-1 membrane glycoprotein; membrane-bound immunoglobulin associated protein; Membrane-bound immunoglobulin-associated protein; signal transduction via sig in B lymphocytes; putative; similar to Homo sapiens CD79A antigen (immunoglobulin-associated alpha) encoded by GenBank Accession Number U05259; Surface IgM-associated protein; unnamed protein product
Gene Aliases: CD79A; Ig-alpha; IGA; IGAlpha; Ly-54; Ly54; MB-1; MB1
UniProt ID: (Human) P11912, (Mouse) P11911
Entrez Gene ID: (Human) 973, (Mouse) 12518
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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