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Invitrogen

CD115 (c-fms) Monoclonal Antibody (12-3A3-1B10), eBioscience™

1 Published Figure
4 References
View all (85) CSF1R (CD115) antibodies

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Product Details

14-1159-82

Applications
Tested Dilution
Publications

Western Blot (WB)

Assay-Dependent
-

Immunohistochemistry (IHC)

Assay-Dependent
-

Flow Cytometry (Flow)

1 µg/test
View 3 publications 3 publications

Immunoprecipitation (IP)

Assay-Dependent
-

Miscellaneous PubMed (Misc)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

12-3A3-1B10

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Amount

100 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_493929

Product Specific Information

Description: CD115 or Colony-Stimulating Factor-1 Receptor (CSF-1R) is expressed on cells committed to the monocyte lineage and osteoclasts. CSF-1R is a disulfide-linked homodimer, which upon binding of its ligand, CSF-1, undergoes tyrosine autophosphorylation and subsequently, phosphorylates other membrane-proximal downstream targets which results in cytoskeletal remodeling, gene transcription and protein translation. CSF-1R activation promotes the survival, proliferation and differentiation of mononuclear phagocytes and the spreading and motility of macrophages. In osteoclasts, CSF-1R synergizes with RANKL to regulate the differentiation of mononuclear phagocytes to osteoclasts.

Applications Reported: This 12-3A3-1B10 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistochemical staining.

Applications Tested: This 12-3A3-1B10 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CSF1R (FMS) is involved in the production, differentiation, and function of macrophages, and is implicated in promyelocytic leukemias. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. CSF1R is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in the gene encoding CSF1R have been associated with a predisposition to myeloid malignancy.Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and plays an important role in innate immunity and in inflammatory processes. The first intron of the CSF1R gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction. Mutations in the CSF1R gene have been associated with a predisposition to myeloid malignancy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CD115; CD115 antigen; CSF-1 receptor; CSF-1-R; CSFIR; EC 2.7.10.1; FMS proto-oncogene; kinase CSFR; macrophage colony stimulating factor I receptor; Macrophage colony-stimulating factor 1 receptor; McDonough feline sarcoma viral (v-fms) oncogene homolog; OTTHUMP00000224093; Proto-oncogene c-Fms; transmembrane protein; unnamed protein product

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Gene Aliases: BANDDOS; C-FMS; CD115; CSF-1R; CSF1R; CSFR; FIM2; FMS; GPSC; HDLS; HDLS1; M-CSF-R

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UniProt ID: (Human) P07333

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Entrez Gene ID: (Human) 1436

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Function(s)
nucleotide binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity macrophage colony-stimulating factor receptor activity protein binding ATP binding kinase activity transferase activity growth factor binding protein phosphatase binding cytokine binding protein homodimerization activity
Process(es)
positive regulation of protein phosphorylation immune system process myeloid leukocyte differentiation response to ischemia response to stress inflammatory response signal transduction transmembrane receptor protein tyrosine kinase signaling pathway axon guidance cell proliferation positive regulation of cell proliferation negative regulation of cell proliferation regulation of cell shape anatomical structure morphogenesis positive regulation of macrophage chemotaxis cell migration peptidyl-tyrosine phosphorylation cytokine-mediated signaling pathway olfactory bulb development forebrain neuron differentiation regulation of cell morphogenesis hemopoiesis monocyte differentiation macrophage differentiation osteoclast differentiation positive regulation of cell migration ruffle organization positive regulation of chemokine production regulation of actin cytoskeleton organization cellular response to macrophage colony-stimulating factor stimulus macrophage colony-stimulating factor signaling pathway positive regulation of tyrosine phosphorylation of STAT protein negative regulation of apoptotic process regulation of MAPK cascade positive regulation by host of viral process innate immune response regulation of bone resorption cell-cell junction maintenance protein autophosphorylation animal organ development positive regulation of protein kinase B signaling mammary gland duct morphogenesis positive regulation of protein tyrosine kinase activity microglial cell proliferation positive regulation of ERK1 and ERK2 cascade cellular response to cytokine stimulus positive regulation of macrophage proliferation regulation of macrophage migration positive regulation of cell motility
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