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Invitrogen
Trefoil Factor 3 Monoclonal Antibody (5UCLNT3), eBioscience™
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FIGURE: 1 / 1
Trefoil Factor 3 Antibody (14-4758-82) in IHC (P)
Immunohistochemistry of formalin-fixed paraffin embedded rat small intestine tissue using 0.5 µg/mL of Anti-Mouse/Rat Trefoil Factor 3 Purified followed by Anti-Mouse IgG Biotin, Streptavidin-HRP, and DAB visualization. Nuclei are counterstained with hematoxylin.
Product Details
14-4758-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
5UCLNT3
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The monoclonal antibody 5UCLNT3 recognizes mouse and rat Trefoil Factor 3. Trefoil Factor 3 (TFF3) is a member of the trefoil family, which are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. TFF3 is a small peptide hormone secreted by mucus-producing cells and by epithelial cells of multiple tissues, including the gastrointestinal tract, kidney, and breast. TFF3 plays an essential role in both mucosal surface maintenance and restitution. TFF3 facilitates restoration of the intestinal epithelium as a protective barrier against injury by inhibiting apoptosis and promoting survival and migration of epithelial cells. TFF3 also plays a role in inducing airway epithelial ciliated cell differentiation. Levels of TFF3 change after renal injury and urinary levels can be used as a biomarker for kidney tubular injury. TFF3 may also play a role in breast tumor progression and tumor cell dissemination.
Applications Reported: This 5UCLNT3 antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and microscopy.
Applications Tested: This 5UCLNT3 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded rat tissue using low pH antigen retrieval and can be used at less than or equal to 0.5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing. Trefoil peptides contain three intrachain disulfide bonds, forming the trefoil motif, or P-domain and clustered in human chromosome 21q22.3. Trefoil Factor 3 (TFF3) is one of three trefoil peptides secreted by epithelial cells that line mucus membranes in the large and small intestines and lower respiratory tract. TFF3 is mainly active as a disulfide-linked homodimer and may have a role in promoting cell migration in healing processes. It is involved in the maintenance and repair of the intestinal mucosa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Intestinal trefoil factor; mITF; Polypeptide P1.B; rITF; rP1.B; Trefoil factor 3
Gene Aliases: ITF; mITF; Tff3
UniProt ID: (Mouse) Q62395, (Rat) Q03191
Entrez Gene ID: (Mouse) 21786, (Rat) 25563
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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