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Invitrogen
Amphiregulin Monoclonal Antibody (AREG559), eBioscience™
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FIGURE: 1 / 2
Amphiregulin Antibody (14-5370-82) in WB
Western Blot analysis was performed on whole cell extracts of human peripheral blood cells treated with Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975) overnight (Lane 1) or only with Protein Transport Inhibitor Cocktail (Product # 00-4980) (Lane 2). The blot was blocked with 5% non-fat milk followed by probing with 5 µg/mL of Amphiregulin Monoclonal Antibody (Product # 14-5370-82) . Bands were visualized by chemiluminescence using Rat anti-Mouse IgG Secondary Antibody, HRP followed by SuperSignal™ West Pi... View More
Product Details
14-5370-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
AREG559
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The AREG858 monoclonal antibody reacts with human Amphiregulin (AR). AR is a member of the Epidermal Growth Factor (EGF) family and is expressed as a transmembrane precursor protein and released by proteolytic cleavage. Soluble AR is a ligand for the EGF receptor (EGFR) and the interaction promotes growth of normal epithelial cells, fibroblasts and keratinocytes. In the context of the immune system AR functions as an effector molecule for tissue repair and homeostasis, mediates resistance and tolerance to infection, and suppresses inflammation. Under different disease and activating conditions AR is expressed in T cells, group 2 innate lymphoid cells, basophils, mast cells, eosinophils, and neutrophils.
Applications Reported: This AREG559 antibody has been reported for use in flow cytometric analysis and western blot.
Applications Tested: This AREG559 antibody has been tested by western blot analysis of stimulated normal human peripheral blood cells. This can be used 1-10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Target Information
Amphiregulin (AR) binds to EGF-Receptor (EGFR) with lower affinity than EGF. The mature secreted form AR is an 84-amino acid residue glycosylated polypeptide growth regulator, which is generated by proteolytic processing of a 252-amino acid transmembrane precursor. Seven different polypeptide ligands, which derive from distinct genes, are capable of binding to the extracellular domain of EGFR. These ligands include EGF, TGFalpha, AR, HB-EGF, cripto-1, epiregulin, and betacellulin. All of these growth factors contain a characteristic EGF-like domain which is defined by 6 evenly spaced cysteine residues that generate 3 loops through the formation of disulfide bonds. AR protein has been localized by immunohistochemistry to the epithelium of the colon, stomach, pancreas, breast, and placenta. AR is reportedly overexpressed in human cancers of breast, colon, stomach, and pancreas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Amphiregulin; amphiregulin B; AR; Colorectum cell-derived growth factor; CRDGF; MGC13647; schwannoma-derived growth factor
Gene Aliases: AR; AREG; AREGB; CRDGF; SDGF
UniProt ID: (Human) P15514
Entrez Gene ID: (Human) 374
epidermal growth factor receptor signaling pathway
G-protein coupled receptor signaling pathway
cell-cell signaling
positive regulation of cell proliferation
positive regulation of keratinocyte proliferation
glial cell proliferation
neuron projection development
response to estradiol
ERBB2-EGFR signaling pathway
positive regulation of phosphorylation
response to hydrogen peroxide
response to peptide hormone
negative regulation of osteoblast differentiation
response to glucocorticoid
response to cAMP
dichotomous subdivision of terminal units involved in mammary gland duct morphogenesis
mammary gland branching involved in thelarche
mammary gland alveolus development
epithelial cell proliferation involved in mammary gland duct elongation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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