Invitrogen

Actin (muscle) Monoclonal Antibody (HHF35), eBioscience™

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (1)
Published Figures (2)

Actin (muscle) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 3

Actin (muscle) Antibody (14-6496-82) in IHC (P)

Immunohistochemistry on formalin-fixed paraffin embedded human pancreas using 10 µg/mL of Mouse IgG1 Isotype Control Purified (Product # 14-4714-82) (left) or 10 µg/mL Anti-Actin (muscle) Purified (right) followed by biotinylated Anti-Mouse IgG, and DAB visualization.Nuclei are counterstained with hematoxylin.

Actin (muscle) Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

14-6496-82

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL

Immunohistochemistry (Frozen) (IHC (F))

5 µg/mL

Immunocytochemistry (ICC/IF)

Assay-Dependent

Product Specifications

Species Reactivity

Bovine, Dog, Horse, Guinea pig, Human, Mouse, Non-human primate, Pig, Rat

Published species

Not Applicable

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

HHF35

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11044624

Product Specific Information

Description: The monoclonal antibody HHF35 recognizes muscle actin; specifically the alpha and gamma form found in skeletal cardiac, and smooth muscle. No reactivity to the beta form is observed. The 42 kDa protein is a main component of the cytoskeletal structural network. The HHF35 antibody has been shown to recognize actin from smooth muscle cells of the heart, bladder, uterus, gastrointestinal tract, and diaphragm. The antibody stains tumors of muscle origin such as leiomyomas, leiosarcomes and rhabdomyosacromas.

The HHF35 antibody recognizes human, mouse, rat, bovine, pig, guinea pig, sheep, frog, canine and non-human primate actin.

Applications Reported: This HHF35 antibody has been reported for use in immunohistochemical staining of frozen and formalin-fixed paraffin embedded tissue sections and western blotting.

Applications Tested: This HHF35 antibody has been tested by immunohistochemistry on FFPE human pancreas (with IHC Antigen Retrieval Solution - Low pH (Product # 00-4955-58) and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Restoration of Corneal Transparency by Mesenchymal Stem Cells.

Stem cell reports

Mittal SK,Omoto M,Amouzegar A,Sahu A,Rezazadeh A,Katikireddy KR,Shah DI,Sahu SK,Chauhan SK

Published figure using Actin (muscle) monoclonal antibody (Product # 14-6496-82) in Immunofluorescence

Tue Oct 11 00:00:00 EDT 2016

Morphological characterisation of portal myofibroblasts and hepatic stellate cells in the normal dog liver.

Comparative hepatology

Ijzer J,Roskams T,Molenbeek RF,Ultee T,Penning LC,Rothuizen J,van den Ingh TS

Published figure using Actin (muscle) monoclonal antibody (Product # 14-6496-82) in Immunohistochemistry

Thu Nov 16 00:00:00 EST 2006

Bioinformatics

Protein Aliases: ACTB; actin; actin alpha 1; actin alpha cardiac; actin alpha cardiac 1; actin homolog; actin, alpha 1, skeletal muscle; actin, alpha 2, smooth muscle, aorta; Actin, alpha cardiac muscle 1; Actin, alpha skeletal muscle; actin, alpha, cardiac 1; actin, alpha, vascular smooth muscle; Actin, aortic smooth muscle; Actin, cytoplasmic 2; actin, gamma 2, smooth muscle, enteric; actin, gamma, cytoplasmic 1; Actin, gamma-enteric smooth muscle; actin-like protein; alpha actin; alpha actin 1; alpha actin 2; alpha-actin cardiac; Alpha-actin-1; Alpha-actin-2; Alpha-actin-3; Alpha-cardiac actin; alpha-sma; alpha-smooth muscle actin; alphac-actin; B-actin; beta actin; beta-actin FE-3; cardiac actin; cardiac alpha actin; cardiac muscle alpha actin 1; Cell growth-inhibiting gene 46 protein; cytoplasmic actin; cytoskeletal gamma-actin; deafness, autosomal dominant 20; deafness, autosomal dominant 26; epididymis luminal protein 176; Gamma-2-actin; Gamma-actin; gamma-enteric smooth muscle actin; nemaline myopathy type 3; RP5-1068B5.2; skeletal alpha actin; skeletal muscle alpha-actin 1; smooth muscle alpha-actin; Smooth muscle gamma-actin; vascular smooth muscle alpha-actin

View more View less

Gene Aliases: 0610041G09Rik; a-SMA; AA959943; AAT6; ACT; ACT-4; Act4; ACTA; Acta-2; ACTA1; ACTA2; ACTA3; ACTC; Actc-1; ACTC1; ACTE; ACTG; ACTG1; ACTG2; ACTGE; actin; Actl; ACTL3; Acts; ACTSA; ACTSG; Actsk-1; ACTVS; AL023024; alphaSMA; ASD5; ASMA; BRWS2; CFTD; CFTD1; CFTDM; CMD1R; CMH11; DFNA20; DFNA26; E51; GIG46; HEL-176; LVNC4; MPFD; MYMY5; NEM1; NEM2; NEM3; SHPM; SMalphaA; SMGA; VSCM