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Invitrogen
c-Met Monoclonal Antibody (eBioclone 7), eBioscience™
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FIGURE: 1 / 7
c-Met Antibody (14-8854-82) in Flow
Staining of mouse c-Met-transfected 293T cells with 0.5 µg of Rat IgG1 K Isotype Control Purified (Product # 14-4301-82) (open histogram) or 0.5 µg of Anti-Mouse c-Met (HGF Receptor) Purified (filled histogram) followed by F (ab')2 Anti-Rat IgG PE (Product # 12-4822).Total viable cells were used for analysis.
Product Details
14-8854-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
eBioclone 7
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The eBioclone 7 monoclonal antibody was generated against a mouse c-Met-Ig fusion protein, and reacts with mouse c-Met-transfected cells. Mouse c-Met (HGFR) is a 170 kDa receptor tyrosine kinase (RTK) expressed by epithelial cells of the brain, kidney, liver and other tissues. Binding of its ligand, Hepatocyte Growth Factor (HGF), triggers receptor autophosphorylation, and activation of several downstream effectors including the mitogen-activated protein kinases ERK-1 and ERK-2, and PLC gamma. Activation of the c-Met signal transduction pathway leads to mulitple cellular responses including cell motility, scattering, proliferation, survival and angiogenesis. Mutations in human c-Met have been implicated in the development of several malignancies.
Applications Reported: This eBioclone 7 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBioclone 7 antibody has been tested by flow cytometric analysis of mouse c-Met-transfected 293T cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
MET (cMET) is a receptor-like tyrosine kinase whose dysregulation has been linked to many types of human malignancies. After activation of the ligand, MET interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, and STAT3. There interactions lead to the activation of signaling cascades including RAS-ERK, PI3, kinase-AKT, and PLCgamma-PKC. MET plays a role in embryonic development including gastrulation, development of muscles and neurons, angiogenesis, and kidney formation. It also plays a role in adults including wound healing, organ regeneration, and tissue remodeling. MET has been linked to cancers including gastric, renal, and breast; therefore, making it a target for cancer therapeutics and diagnostic testing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cMet; EC 2.7.10.1; hepatocyte growth factor receptor; HGF receptor; HGF-SF receptor; HGF/SF receptor; met proto-oncogene tyrosine kinase; oncogene MET; proto-oncogene c-Met; sc MET; scatter factor receptor; SF receptor; soluble c met; tyrosine-protein kinase Met
Gene Aliases: AI838057; c-Met; HGF; HGFR; Par4
Entrez Gene ID: (Mouse) 17295
nucleotide binding
protein kinase activity
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
hepatocyte growth factor-activated receptor activity
protein binding
ATP binding
beta-catenin binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
protein phosphatase binding
protein complex binding
phospholipase binding
phosphatidylinositol 3-kinase binding
protein heterodimerization activity
transmembrane signal receptor
negative regulation of transcription from RNA polymerase II promoter
activation of MAPK activity
neuron migration
endothelial cell morphogenesis
liver development
placenta development
protein phosphorylation
transmembrane receptor protein tyrosine kinase signaling pathway
brain development
muscle organ development
skeletal muscle tissue development
positive regulation of gene expression
positive regulation of peptidyl-threonine phosphorylation
negative regulation of peptidyl-threonine phosphorylation
positive regulation of glucose transport
positive regulation of neuron projection development
muscle cell migration
myotube differentiation
phosphorylation
peptidyl-tyrosine phosphorylation
sperm motility
adult behavior
positive regulation of peptidyl-serine phosphorylation
glucose homeostasis
positive regulation of DNA replication
positive regulation of mitotic nuclear division
positive regulation of transcription from RNA polymerase II promoter
protein autophosphorylation
hepatocyte growth factor receptor signaling pathway
branching morphogenesis of an epithelial tube
positive regulation of dendrite morphogenesis
modulation of synaptic transmission
positive chemotaxis
myoblast proliferation
excitatory postsynaptic potential
regulation of branching involved in salivary gland morphogenesis by mesenchymal-epithelial signaling
semaphorin-plexin signaling pathway
negative regulation of hydrogen peroxide-mediated programmed cell death
positive regulation of endothelial cell chemotaxis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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