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Phospho-BTK/ITK (Tyr551, Tyr511) Antibody (14-9015-82) in IHC (P)
Immunohistochemistry of formalin-fixed paraffin embedded human tonsil using 0.5 µg/mL of Anti-Human/Mouse Phospho-BTK/ITK (Y551/Y511) Purified, followed by Anti-Mouse IgG Biotin, Streptavidin HRP and DAB visualization.Nuclei are counterstained with hematoxylin.
Product Details
14-9015-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Mouse
/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
M4G3LN
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This M4G3LN monoclonal antibody recognizes human and mouse Bruton's tyrosine kinase (also known as BTK, agammaglobulinaemia tyrosine kinase, B-cell progenitor kinase)/interleukin-2-inducible tyrosine kinase (also known as ITK, T-cell-specific kinase, Tyrosine-protein kinase Lyk) when phosphorylated on Y551 and Y511, respectively.
In addition to Src family and SYK family kinases, B cells and T cells express a third family of receptor-proximal kinases called the Tec kinase family. The B cell-specific Tec family kinase is BTK; T cells express ITK as well as Tec and Rlk. Tec family kinases play an indispensable role in activation of phospholipase C and activation of calcium-dependent signaling cascades following antigen receptor triggering. BTK has been implicated in propagating signals downstream of LPS-TLR4 in B cells via signaling through the TLR4 co-receptor RP105/CD180. Mice congenitally lacking BTK or ITK show profound defects in B cell or T cell development and signaling, respectively. People with mutations or deletions in BTK suffer from X-linked agammaglobulinemia and X-linked immunodeficiency.
Specificity of this M4G3LN clone was determined by ELISA, flow cytometry, and western blotting.
Applications Reported: This M4G3LN antibody has been reported for use in intracellular staining followed by flow cytometric analysis, western blotting, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. (Fluorochrome conjugated M4G3LN is recommended for use in intracellular flow cytometry.).
Applications Tested: This M4G3LN antibody has been tested by immunoblotting of stimulated Daudi B cells. This can be used at less than or equal to 5 µg/mL. This antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using high pH antigen retrieval and can be used at less than or equal to 0.1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
In addition to Src family and SYK family kinases, B cells and T cells express a third family of receptor-proximal kinases called the Tec kinase family. The B cell-specific Tec family kinase is BTK; T cells express ITK as well as Tec and Rlk. Tec family kinases play an indispensable role in activation of phospholipase C and activation of calcium-dependent signaling cascades following antigen receptor triggering. BTK has been implicated in propagating signals downstream of LPS-TLR4 in B cells via signaling through the TLR4 co-receptor RP105/CD180. Mice congenitally lacking BTK or ITK show profound defects in B cell or T cell development and signaling, respectively. People with mutations or deletions in BTK suffer from X-linked agammaglobulinemia and X-linked immunodeficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: agammaglobulinaemia tyrosine kinase; Agammaglobulinemia tyrosine kinase; ATK; B-cell progenitor kinase; BPK; Bruton agammaglobulinemia tyrosine kinase; Bruton tyrosine kinase; Bruton's tyrosine kinase; dominant-negative kinase-deficient Brutons tyrosine kinase; homolog of mouse T-cell itk/tsk; IL-2-inducible T cell kinase; IL-2-inducible T-cell kinase; IL2-inducible T-cell kinase; interleukin-2-inducible T cell kinase; Interleukin-2-inducible T-cell kinase; Kinase EMB; Kinase EMT; Kinase TLK; T-cell-specific kinase; truncated Bruton agammaglobulinemia tyrosine kinase; Tyrosine-protein kinase BTK; Tyrosine-protein kinase BTK (Brutons tyrosine kinase) (Agammaglobulinaemia tyrosine kinase) (ATK) (B cell progenitor kinase) (BPK) (Kinase EMB); tyrosine-protein kinase BTK isoform (lacking exon 13 to 17); tyrosine-protein kinase BTK isoform (lacking exon 14); Tyrosine-protein kinase ITK/TSK; Tyrosine-protein kinase Lyk; X-linked immune deficiency
Gene Aliases: AGMX1; AI528679; AT; ATK; BPK; BTK; EMT; IMD1; ITK; LPFS1; LYK; PSCTK1; PSCTK2; Tcsk; Tlk; Tsk; xid; XLA
UniProt ID: (Human) Q06187, (Mouse) P35991, (Human) Q08881, (Mouse) Q03526
Entrez Gene ID: (Human) 695, (Mouse) 12229, (Human) 3702, (Mouse) 16428
protein tyrosine kinase activity
non-membrane spanning protein tyrosine kinase activity
receptor binding
protein binding
ATP binding
phosphatidylinositol-3,4,5-trisphosphate binding
identical protein binding
metal ion binding
nucleotide binding
protein kinase activity
lipid binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
scaffold/adaptor protein
GTPase-activating protein
negative regulation of cytokine production
adaptive immune response
regulation of B cell cytokine production
MyD88-dependent toll-like receptor signaling pathway
regulation of B cell apoptotic process
transcription, DNA-templated
protein phosphorylation
transmembrane receptor protein tyrosine kinase signaling pathway
I-kappaB kinase/NF-kappaB signaling
mesoderm development
peptidyl-tyrosine phosphorylation
calcium-mediated signaling
intracellular signal transduction
peptidyl-tyrosine autophosphorylation
Fc-epsilon receptor signaling pathway
B cell activation
regulation of cell proliferation
innate immune response
positive regulation of B cell differentiation
cell maturation
B cell receptor signaling pathway
positive regulation of NF-kappaB transcription factor activity
apoptotic signaling pathway
immune system process
histamine secretion by mast cell
regulation of transcription, DNA-templated
apoptotic process
response to organic substance
phosphorylation
cell differentiation
cellular response to reactive oxygen species
protein autophosphorylation
cellular response to molecule of fungal origin
cytokine production
NK T cell differentiation
cellular defense response
signal transduction
activation of phospholipase C activity
interferon-gamma production
interleukin-4 production
T cell activation
T cell receptor signaling pathway
Performance Guarantee
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