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Invitrogen
Desmin Monoclonal Antibody (DE-U-10), eBioscience™
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FIGURE: 1 / 17
Desmin Antibody (14-9747-82) in ICC/IF
Immunofluorescence analysis Desmin was performed using 70% confluent log phase RD cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Desmin Monoclonal Antibody (DE-U-10), eBioscience™ (Product 14-9747-82) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 (Product # A28175)... View More
Product Details
14-9747-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Chicken,
Cat,
Goat,
Hamster,
Human,
Mouse,
Sheep,
Pig,
Rabbit,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
DE-U-10
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The monoclonal antibody DE-U-10 recognizes desmin. Desmin is the protein subunit of class-III intermediate filaments. Desmin is found predominantly in skeletal, cardiac, and smooth muscle. Desmin plays a role in muscle cell development and differentiation, muscle cell architecture and structure, and mitochondrial function. Desmin forms scaffolds around the Z-disk of sarcomeres in muscle cells. Desmin knockout mice exhibit defects in skeletal, smooth, and cardiac muscle and have impaired mitochondrial function. Mutations in Desmin result in conditions such as desmin-related myopathy (DRM), cardiomyopathy dilated type 1I (CMD1I), and neurogenic scapuloperoneal syndrome Kaeser type (Kaeser syndrome).
The DE-U-10 antibody recognizes human, mouse, rat, feline, bovine, goat, hamster, sheep, porcine, rabbit, and chicken desmin. The DE-U-10 antibody will also label tumors derived from muscle tissue, such as leiomyomas and rhabdomyosarcomas.
Applications Reported: This DE-U-10 antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry.
Applications Tested: This DE-U-10 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using either low or high pH antigen retrieval and can be used at less than or equal to 5 µg/mL. This DE-U-10 antibody has been tested by immunocytochemistry of methanol-fixed and permeabilized mouse cells and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Desmin (approximately 53 kDa) exhibits a high degree of tissue specificity, its expression being predominantly confined to all types of muscle cells (cardiac, skeletal and smooth muscle). Regulation of desmin expression is stage and tissue-specific, since it is induced during terminal development of, for example, skeletal muscle cell differentiation. In skeletal en cardiac muscle cells desmin is localized in the Z-disk region and at the intercalated disk. The expression pattern of desmin in smooth muscle is much more heterogenous. Coexpression of vimentin and desmin has been observed in tumors derived from muscle tissue, i.e. rhabdomyosarcomas and leiomyosarcomas. Furthermore, during myocard dysfunction dramatic changes in the distribution of desmin have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CMD1I; Desmin; desmin-like protein; FLJ12025; FLJ39719; FLJ41013; FLJ41793; intermediate filament protein; MFM1; muscle-specific intermediate filament desmin; muscle-specific intermediate filament protein; mutant desmin p.K241E; SCPNK
Gene Aliases: CSM1; CSM2; DES; I79_018932; LGMD2R
UniProt ID: (Bovine) O62654, (Pig) P02540, (Human) P17661, (Rat) P48675, (Mouse) P31001
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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