Connexin 32 Monoclonal Antibody (Connexin32 (2A)), eBioscience™

Product Specific Information

Description: The Connexin32 monoclonal antibody recognizes human, mouse, rat, and chicken Connexin 32. Connexins are a family of integral membrane proteins that form gap junction channels, which function to facilitate direct cell-cell communication. Gap junctions are comprised of two hemichannels, or connexons, which are made up of six connexin proteins. Over 20 connexin proteins have been identified and each combine to form either homomeric or heteromeric channels with different functional properties. Connexin 32 is expressed by myelin, neurons, oligodendrocytes, and hepatocytes, and plays a role in the development of ovarian follicles. Connexin 32 expression also plays a role in disease progression in chronic multiple sclerosis and experimental autoimmune encephalomyelitis. Mutations in the Connexin 32 gene, GJB1, result in a form of Charcot-Marie-Tooth disease which is characterized by peripheral nerve dysfunction.

Applications Reported: This Connexin32 antibody has been reported for use in western blotting, immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry.

Applications Tested: This Connexin32 antibody has been tested by immunohistochemistry of frozen mouse tissue and immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and the antibody can be used at less than or equal to 5 µg/mL. This Connexin32 antibody has been tested by immunocytochemistry of methanol-fixed and permeabilized human cells and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This gene encodes a member of the gap junction protein family. The gap junction proteins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Mutations in this gene cause X-linked Charcot-Marie-Tooth disease, an inherited peripheral neuropathy. Alternatively spliced transcript variants encoding the same protein have been found for this gene.