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Invitrogen
Collagen X Monoclonal Antibody (X53), eBioscience™
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FIGURE: 1 / 9
Collagen X Antibody (14-9771-82) in IHC (P)
Immunohistochemistry of formalin-fixed paraffin embedded human infiltrating ductal carcinoma tissue using 10 µg/mL of Mouse IgG1 K Isotype Control Purified (left) or 10 µg/mL of Anti-Collagen Type X Purified (right) followed by Anti-Mouse IgG Biotin, Streptavidin HRP, and DAB visualization.Nuclei are counterstained with hematoxylin.
Product Details
14-9771-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Guinea pig,
Human,
Mouse,
Pig,
Rat
Published species
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
X53
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The monoclonal antibody X53 recognizes human, mouse, porcine, guinea pig, and rat Collagen Type X. X53 does not cross-react with collagen types I, II, III, IV, or VI. Collagen Type X is a homotrimeric short chain collagen. Collagen Type X has limited expression in normal adult tissues, primarily being expressed during bone growth and development. Expression is localized to the peri- and extracellular matrix of hypertrophic chondrocytes during endochondral ossification and also plays a role in cartilage mineralization. Collagen Type X is elevated in many tumor types with expression being localized in the vasculature. Mutations to the gene COL10A1, which encodes human Collagen Type X, is associated with Schmid type metaphyseal chondrodysplasia.
Applications Reported: This X53 antibody has been reported for use in microscopy, immunohistochemical staining, ELISA, and immunocytochemistry.
Applications Tested: This X53 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Type X collagen is a product of hypertrophic chondrocytes and has been localized to presumptive mineralization zones of hyaline cartilage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Collagen alpha-1(X) chain; collagen X, alpha-1 polypeptide; collagen, type X, alpha 1; collagen, type X, alpha 1(Schmid metaphyseal chondrodysplasia); pro alpha 1; procollagen, type X, alpha 1; Schmid metaphyseal chondrodysplasia; type X collagen; type X collagen, alpha 1
Gene Aliases: Col10; Col10a-1; COL10A1
UniProt ID: (Human) Q03692, (Mouse) Q05306
Entrez Gene ID: (Pig) 448809, (Human) 1300, (Dog) 100856711, (Mouse) 12813, (Rat) 25681, (Guinea pig) 106026924
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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