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Invitrogen
CD227 (Mucin 1) Monoclonal Antibody (SM3), eBioscience™
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FIGURE: 1 / 1
CD227 (Mucin 1) Antibody (14-9893-82) in IHC (P)
Immunohistochemistry of formalin-fixed paraffin embedded human infiltrating ductal carcinoma, using 5 µg/mL of Mouse IgG1 Isotype Control Purified (Product # 14-4714-82) (left) or Anti-Human CD227 (Mucin 1) Purified (right) followed by Anti-Mouse Biotin, and DAB visualization.Nuclei are counterstained with hematoxylin.
Product Details
14-9893-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
SM3
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This SM3 monoclonal antibody reacts with the under-glycosylated form of human Mucin 1 (MUC1, CD227), a large glycoprotein belonging to the mucin protein family. Mucin 1 contains a polypeptide core consisting of multiple tandem repeats that become highly glycosylated. Mucin 1 is typically expressed in ductal or glandular epithelial cells and is localized to the apical membrane. In cancerous cells, Mucin 1 expression is increased and membrane-specific localization is lost resulting in expression throughout the membrane and cytoplasm. High levels of under-glycosylated Mucin 1 are thought to affect cell behavior during both invasion and metastasis as well as in immune recognition. In addition, under-glycosylated Mucin 1 is shed from the epithelial cell surface and can be detected in circulation. Alterations in Mucin 1 glycosylation are found in most adenocarcinomas of the breast, lung, pancreas, prostate, and ovary. Mucin 1 has recently been shown to co-localize and interact with members of the erbB receptor kinase family, proteins that are upregulated in more aggressive forms of breast cancer.
Please note this antibody sees a distinct epitope from other Mucin 1 antibodies.
Applications Reported: This SM3 antibody has been reported for use in western blotting, immunohistochemical staining, and immunocytochemistry.
Applications Tested: This SM3 antibody has been tested by western blot and immunocytochemistry on MCF7 cell line as well as by immunohistochemistry on FFPE (formalin-fixed paraffin embedded) tissue. This can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
MUC1 (Mucin 1, Episialin, MAM-6, CA 15-3, PEM and EMA) is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. MUC1 is a transmembrane glycoprotein with a large mucin-like extracellular domain that matures through several intermediate forms generated by proteolysis, and sequential addition and processing of numerous O-linked glycans that are heavily sialylated. MUC1 is highly polymorphic and each allele encodes a product that contains a different number of repeats (between 30 and 90) leading to large differences in molecular weight of the protein. MUC1 is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells, and is expressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four cebB receptors and localize with erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. Overexpression, aberrant intracellular localization, and changes in glycosylation of the MUC1 protein have been associated with carcinomas. Multiple alternatively spliced transcript variants that encode different isoforms of the MUC1 gene have been reported, but the full-length nature of only some has been determined. Transgenic MUC-1 has been shown to associate with all four cebB receptors and localize with erbB1 (EGFR) in lactating glands.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Breast carcinoma-associated antigen DF3; CA 15-3; Cancer antigen 15-3; Carcinoma-associated mucin; CD227; cell membrane protein; DF3 antigen; EMA; Episialin; H23 antigen; H23AG; J19; KL-6; Krebs von den Lungen-6; MUC-1; MUC1; MUC1-alpha; MUC1/E1; transmembrane; devoid of tandem repeats; MUC1/E2; transmembrane; devoid of tandem repeats; longer signal peptide; MUC1/F; transmembrane; MUC1/G; transmembrane; devoid of tandem repeats; MUC1/PC1; MUC1/PC2; MUC1/S1; potential secreted protein; MUC1/S2; potential secreted protein; MUC1/S; potential secreted protein with no tandem repeats; MUC1/SV1; MUC1/SV10; MUC1/SV2; MUC1/SV3; MUC1/SV4; MUC1/SV7; MUC1/SV8; MUC1/SV9; mucin 1, transmembrane; mucin glycoprotein; mucin short variant Y; transmembrane; devoid of tandem repeats; Mucin-1; Peanut-reactive urinary mucin; PEM; PEMT; Polymorphic epithelial mucin; PUM; short variant; tumor associated epithelial mucin; Tumor-associated epithelial membrane antigen; Tumor-associated mucin; Unknown; unnamed protein product
Gene Aliases: ADMCKD; ADMCKD1; ADTKD2; CA 15-3; Ca15-3; CD227; EMA; H23AG; KL-6; MAM6; MCD; MCKD; MCKD1; MUC-1; MUC-1/SEC; MUC-1/X; MUC1; MUC1/ZD; PEM; PEMT; PUM
UniProt ID: (Human) P15941
Entrez Gene ID: (Human) 4582
negative regulation of transcription by competitive promoter binding
DNA damage response, signal transduction by p53 class mediator
mitotic G1 DNA damage checkpoint
negative regulation of cell adhesion mediated by integrin
positive regulation of transcription from RNA polymerase II promoter
negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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