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Invitrogen

CD51/CD61 (Integrin alpha v beta 3) Monoclonal Antibody (23C6), Functional Grade, eBioscience™

1 Published Figure
3 References
View all (12) CD51/CD61 antibodies

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Product Details

16-0519-81

Applications
Tested Dilution
Publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent
-

Immunocytochemistry (ICC/IF)

-
View 1 publication 1 publication

Flow Cytometry (Flow)

1 µg/test
-

Neutralization (Neu)

Assay-Dependent
-

Functional Assay (Functional)

Assay-Dependent
View 1 publication 1 publication

Inhibition Assays (Inhibition)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

23C6

Conjugate

Functional Grade Functional Grade Functional Grade

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_529538

Product Specific Information

Description: The 23C6 monoclonal antibody reacts with the human CD51/CD61 dimer, also known as the integrin alphav/beta3. CD51, an ~120 kDa surface molecule can also non-covalently associate with other beta subunits of the integrin family including beta1 (CD29), beta5 and beta6 to form receptors for extracellular matrix components. Heterodimers of CD51/CD61 are expressed by melanoma cells, endothelial cells and osteoclasts and at very low levels by platelets. The CD51/CD61 complex mediates adhesion to fibrinogen, fibronectin, vitronectin and thrombospondin.

Applications Reported: This 23C6 antibody has been reported for use in flow cytometric analysis, and immunohistology staining of frozen tissue sections. 23C6 has also been reported in blocking of some adhesive processes.

Applications Tested: This 23C6 antibody has been tested by flow cytometric analysis of human melanoma cell line and peripheral blood. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

CD51 and CD61 molecules (alpha V and beta 3 integrins) form a complex. This complex binds vitronection at the RGD sequence and can also bind fibrinogen, von Willebrand factor, thrombospondin, fibronectin, osteopontin and collagen. Reacts with osteoclasts, placenta, melanoma cell lines and weakly with platelets.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: antigen CD61; antigen identified by monoclonal antibody L230; CD51; CD61; glycoprotein IIIa precursor; GPIIIa; integrin alpha-5 beta-3; Integrin alpha-V; integrin alphaVbeta3; integrin beta 3; integrin beta chain, beta 3; Integrin beta-3; integrin beta-3 subunit; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61); plate glycoprotein IIIa (GPIIIa); Platelet membrane glycoprotein IIIa; unnamed protein product; vitronectin alpha subunit precursor; Vitronectin receptor; Vitronectin receptor subunit alpha

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Gene Aliases: BDPLT16; BDPLT2; BDPLT24; CD51; CD61; GP3A; GPIIIa; GT; GT2; ITGAV; ITGB3; MSK8; VNRA; VTNR

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UniProt ID: (Human) P06756, (Human) P05106

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Entrez Gene ID: (Human) 3685, (Human) 3690

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Function(s)
virus receptor activity opsonin binding fibronectin binding protease binding protein kinase C binding receptor binding integrin binding protein binding coreceptor activity fibroblast growth factor binding C-X3-C chemokine binding insulin-like growth factor I binding signaling receptor activity neuregulin binding metal ion binding transforming growth factor beta binding extracellular matrix binding extracellular matrix protein binding protein disulfide isomerase activity platelet-derived growth factor receptor binding enzyme binding identical protein binding vascular endothelial growth factor receptor 2 binding cell adhesion molecule binding fibrinogen binding
Process(es)
angiogenesis positive regulation of endothelial cell proliferation positive regulation of cell-matrix adhesion positive regulation of leukocyte migration cell-substrate junction assembly cell adhesion cell-matrix adhesion integrin-mediated signaling pathway embryo implantation blood coagulation regulation of G-protein coupled receptor protein signaling pathway positive regulation of cell proliferation positive regulation of endothelial cell migration positive regulation of gene expression negative regulation of macrophage derived foam cell differentiation positive regulation of fibroblast migration negative regulation of lipid storage negative regulation of low-density lipoprotein particle clearance response to activity smooth muscle cell migration positive regulation of smooth muscle cell migration cell migration platelet activation positive regulation of cell migration positive regulation of vascular endothelial growth factor receptor signaling pathway cell-substrate adhesion negative regulation of lipid transport regulation of protein localization regulation of actin cytoskeleton organization cell adhesion mediated by integrin positive regulation of cell adhesion mediated by integrin positive regulation of osteoblast proliferation heterotypic cell-cell adhesion substrate adhesion-dependent cell spreading tube development wound healing, spreading of epidermal cells response to platelet-derived growth factor cellular response to platelet-derived growth factor stimulus apolipoprotein A-I-mediated signaling pathway wound healing apoptotic cell clearance regulation of bone resorption positive regulation of angiogenesis positive regulation of bone resorption viral entry into host cell platelet-derived growth factor receptor signaling pathway positive regulation of fibroblast proliferation mesodermal cell differentiation positive regulation of smooth muscle cell proliferation negative regulation of lipoprotein metabolic process regulation of developmental process negative chemotaxis regulation of transport regulation of release of sequestered calcium ion into cytosol regulation of serotonin uptake angiogenesis involved in wound healing regulation of biological quality positive regulation of ERK1 and ERK2 cascade platelet aggregation cellular response to mechanical stimulus cellular response to xenobiotic stimulus positive regulation of glomerular mesangial cell proliferation blood coagulation, fibrin clot formation maintenance of postsynaptic specialization structure regulation of postsynaptic neurotransmitter receptor internalization regulation of postsynaptic neurotransmitter receptor diffusion trapping positive regulation of substrate adhesion-dependent cell spreading positive regulation of adenylate cyclase-inhibiting opioid receptor signaling pathway positive regulation of vascular endothelial growth factor signaling pathway regulation of trophoblast cell migration regulation of extracellular matrix organization cellular response to insulin-like growth factor stimulus negative regulation of endothelial cell apoptotic process positive regulation of T cell migration blood vessel development vasculogenesis formation of primary germ layer positive regulation of cytosolic calcium ion concentration tissue development endodermal cell differentiation positive regulation of cell adhesion regulation of phagocytosis positive regulation of small GTPase mediated signal transduction ERK1 and ERK2 cascade calcium ion transmembrane transport transforming growth factor beta production entry into host cell by a symbiont-containing vacuole extrinsic apoptotic signaling pathway in absence of ligand cell-cell adhesion positive regulation of intracellular signal transduction negative regulation of entry of bacterium into host cell negative regulation of extrinsic apoptotic signaling pathway
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