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Invitrogen
CD142 Monoclonal Antibody (HTF-1), Functional Grade, eBioscience™
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Product Details
16-1429-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
HTF-1
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This HTF-1 monoclonal antibody reacts with human CD142, which is also known as Tissue Factor. Expression of this type I transmembrane glycoprotein on endothelial cells, monocytes, macrophages, and platelets can be induced by inflammatory mediators (e.g., LPS, IL-1b, TNFa, PMA, or endotoxin). On the other hand, CD142 is expressed constitutively by some tumor cells (e.g., lung, pancreatic, breast, and colon) and non-immune tissues such as the vasculature, central nervous system, kidney, epithelia, and placenta. Studies have also suggested that CD142 exists as a soluble form that circulates in blood. CD142 initiates blood coagulation by associating with and activating the circulating factors VII and VIIa.
The HTF-1 antibody has been reported to exhibit blocking activity.
Applications Reported: This HTF-1 antibody has been reported for use in functional assays, flow cytometric analysis, western blotting, and immunocytochemistry.
Applications Tested: This HTF-1 antibody has been tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
Target Information
This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD142; coagulation factor; Coagulation factor III; coagulation factor III (thromboplastin, tissue factor); F3; TF; Thromboplastin; Tissue factor; tissue factor precursor; tissue factor versions 1 and 2 precursor; unnamed protein product
Gene Aliases: CD142; F3; TF; TFA
UniProt ID: (Human) P13726
Entrez Gene ID: (Human) 2152
positive regulation of endothelial cell proliferation
activation of plasma proteins involved in acute inflammatory response
activation of blood coagulation via clotting cascade
response to stress
blood coagulation
hemostasis
positive regulation of gene expression
positive regulation of platelet-derived growth factor receptor signaling pathway
protein processing
cytokine-mediated signaling pathway
positive regulation of cell migration
positive regulation of TOR signaling
positive regulation of interleukin-8 production
positive regulation of angiogenesis
positive regulation of positive chemotaxis
positive regulation of endothelial cell apoptotic process
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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