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Invitrogen
CD150 Monoclonal Antibody (9D1), Functional Grade, eBioscience™
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FIGURE: 1 / 2
CD150 Antibody (16-1501-82) in Flow
Staining of C57Bl/6 splenocytes with 0.5 µg of Rat IgG1 kappa Isotype Control Purified (Product # 14-4301-82) (open histogram) or 0.5 µg of Anti-Mouse CD150 Purified (filled histogram) followed by Anti-Rat IgG Biotin (Product # 13-4813-85) and Streptavidin PE (Product # 12-4317-87). Cells in the lymphocyte gate were used for analysis.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
16-1501-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
9D1
Conjugate
Functional Grade
Functional Grade
Functional Grade
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The 9D1 monoclonal antibody reacts with mouse CD150, an ~70 kDa transmembrane glycoprotein also known as Signaling Lymphocyte Activation Molecule (SLAM). CD150 is expressed by T (especially TH1) and B cells and its expression is rapidly upregulated on these cells upon activation. Immature thymocytes and dendritic cells also express this antigen. Signaling through SLAM in T cells induces proliferation and augmentation of the interferon-gamma response. Furthermore, SLAM is thought to play a role in adhesion between the T cell and antigen-presenting cell. 9D1 is reported to be an activating antibody.
Mouse hematopoietic stem cells (HSC) can be identified using SLAM family markers, such as CD150+CD244-CD48-. For this application we recommend the use of antibody clone mShad150 (Product # 12-1502).
Applications Reported: This 9D1 antibody has been reported for use in flow cytometric analysis. 9D1 has also been reported to augment T-cell activation including inducing their proliferation and production of IFN gamma.
Applications Tested: This 9D1 antibody has been tested by flow cytometric analysis of mouse thymic and splenic cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
Target Information
CD150, also known as SLAM (Signaling Lymphocyte Activation Molecule), is a member of the immunoglobulin gene superfamily and plays a crucial role in T cell stimulation and immune cell interactions. It is constitutively expressed on peripheral blood memory T cells, T cell clones, immature thymocytes, and a proportion of B cells. CD150 expression is rapidly upregulated upon activation, particularly on naive T cells, Th1 cells, and activated B cells, which express the membrane-bound, soluble, and cytoplasmic isoforms of SLAM. In T cells, signaling through SLAM induces proliferation and enhances the interferon-gamma response. SLAM is also involved in adhesion between T cells and antigen-presenting cells, facilitating immune cell interactions. Homophilic SLAM-SLAM binding during B to B and B to T cell interactions is suggested to enhance the expansion and differentiation of activated B cells. CD150 serves as a receptor for the measles virus and acts as a co-activator on T and B cells. It interacts with SH2D1A and PTPN11 via its cytoplasmic domain, playing a role in signal transduction. Mutations in the CD150 gene may be associated with X-linked lymphoproliferative disease (XLP), highlighting its importance in immune regulation and disease pathology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD150 antigen; CDw150; signaling lymphocyte activation molecule; SLAM; SLAM family member 1; unnamed protein product
Gene Aliases: 4933415F16; CD150; CDw150; ESTM51; IPO-3; Slam
UniProt ID: (Mouse) Q9QUM4
Entrez Gene ID: (Mouse) 27218
natural killer cell differentiation
natural killer cell proliferation
negative regulation of cytokine production
leukocyte chemotaxis involved in inflammatory response
myeloid dendritic cell activation involved in immune response
negative regulation of T cell cytokine production
immune response
positive regulation of cell proliferation
positive regulation of macrophage chemotaxis
regulation of vesicle fusion
negative regulation of interferon-gamma production
negative regulation of interleukin-12 production
negative regulation of interleukin-6 production
negative regulation of tumor necrosis factor production
positive regulation of interferon-gamma production
positive regulation of activated T cell proliferation
leukocyte activation
positive regulation of JNK cascade
lymphocyte activation
viral entry into host cell
regulation of catalytic activity
positive regulation of ERK1 and ERK2 cascade
negative regulation of CD40 signaling pathway
positive regulation of dendritic cell chemotaxis
positive regulation of T-helper 1 cell cytokine production
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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