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Invitrogen
CD226 (DNAM-1) Monoclonal Antibody (10E5), Functional Grade, eBioscience™
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FIGURE: 1 / 2
CD226 (DNAM-1) Antibody (16-2261-81) in Flow
Staining of 3-day Anti-Mouse CD3/CD28-stimulated C57Bl/6 splenocytes with 0.25 µg of Rat IgG2b Kisotype Control Functional Grade Purified (Product # 16-4031-81) (left) or 0.25 µg of Anti-Mouse CD226 Functional Grade Purified (right) followed by Anti-Rat IgG Biotin (Product # 13-4813-85) and Streptavidin PE (Product # 12-4317-87) and subsequently with Anti-Mouse CD8a FITC (Product # 11-0081-82).Total viable cells were used for analysis.
Product Details
16-2261-81
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
10E5
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
50 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This 10E5 monoclonal antibody reacts with mouse CD226, which is also known as DNAX accessory molecule-1 (DNAM-1). CD226 is an adhesion molecule expressed primarily on T cells, including thymocytes. The highest levels of CD226 are found on naive CD8 T cells. Moreover, expression of CD226 is regulated differentially in CD4 T cells. Under certain culture conditions, CD226 can be upregulated in Th1 and downregulated in Th2 and Th0 cells. This cell surface receptor can also be detected on natural killer cells, some B cell subsets, monocytes, and platelets. Interaction with its ligands, the poliovirus receptor (CD155) and CD112, results in adhesion and cell signaling events that promote innate and adaptive immunity, including differentiation, survival, and cytotoxicity.
The 10E5 monoclonal antibody has been reported to inhibit antigen-specific T cell proliferation and IFN-gamma production in vivo.
Applications Reported: This 10E5 antibody has been reported for use in flow cytometric analysis and functional assays.
Applications Tested: This 10E5 antibody has been tested by flow cytometric analysis of 3-day CD3/CD28-stimulated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
Target Information
DNAM 1 is a receptor expressed by peripheral blood T lymphocytes that is involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T lymphocytes. DNAM 1 is broadly expressed on T cells, NK cells, platelets, monocytes and a subset of B cells. DNAM-1 is also expressed by a subset of CD3 positive thymocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD226; CD226 antigen; DNAM 1; platelet and T cell activation antigen 1; Platelet and T-cell activation antigen 1
Gene Aliases: BC051526; Cd226; DNAM-1; DNAM1; Pta1; TLiSA1
UniProt ID: (Mouse) Q8K4F0
Entrez Gene ID: (Mouse) 225825
cytokine production
positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target
positive regulation of immunoglobulin mediated immune response
cell adhesion
positive regulation of mast cell activation
positive regulation of natural killer cell mediated cytotoxicity
positive regulation of Fc receptor mediated stimulatory signaling pathway
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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