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Invitrogen
CD272 (BTLA) Monoclonal Antibody (MIH26), Functional Grade, eBioscience™
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-Functional-Grade-Flow-20170216130641.jpg?time=20220826 "CD272 (BTLA) Antibody in Flow Cytometry (Flow)")
FIGURE: 1 / 1
CD272 (BTLA) Antibody (16-5979-82) in Flow
Staining of normal human peripheral blood cells with 0.25 µg of Mouse IgG2a kappa Isotype Control Purified (Product # 14-4724-82) (open histogram) or 0.25 µg of Anti-Human CD272 (BTLA) Biotin (filled histogram) followed by Anti-Mouse IgG FITC (Product # 11-4011-85). Cells in the lymphocyte gate were used for analysis.
-Functional-Grade-Flow-20170216130641.jpg?time=20220826)
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
-Functional-Grade-Flow-20170216130641.jpg?time=20220826?time=20220826 "CD272 (BTLA) Antibody (16-5979-82) in Flow")
Product Details
16-5979-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Mouse
Host/Isotype
Mouse
/ IgG2a
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
MIH26
Conjugate
Functional Grade
Functional Grade
Functional Grade
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The BTLA monoclonal antibody reacts with human B and T lymphocyte attenuator (BTLA). BTLA is expressed by peripheral lymphocytes, splenic macrophages, developing B cells in the bone marrow and developing T cells in the thymus and mature, but not immature bone marrow-derived dendritic cells. BTLA is implicated as a negative regulator of the activation and/or function of various hemopoietic cell types. It is reported that BTLA binds to B7-H4, but further studies are needed to confirm this interaction.
Applications Reported: This MIH26 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This MIH26 antibody has been tested by flow cytometric analysis of normal human peripheral blood. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
Target Information
BTLA or B and T-lymphocyte attenuator is a member of the co-inhibitory receptors of the CD28 superfamily. BTLA is a lymphocyte inhibitory receptor which inhibits lymphocytes during immunes responses. BTLA is constitutively expressed on most CD4+ and CD8+ T cells and its expression progressively decreases upon T cell activation. It remains expressed on Th1 cells, but not Th2 cells. BTLA is a unique co-receptor that interacts with the tumor necrosis factor receptor superfamily member herpesvirus entry mediator (HVEM). This interation is an important pathway regulating lymphocyte activation and/or homeostasis in the immune response.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: B and T lymphocyte associated; B and T lymphocyte associated protein; B- and T-lymphocyte-associated protein; BTLA; BTLA_HUMAN; CD272 antigen; FLJ16065; MGC129743; unnamed protein product
Gene Aliases: BTLA1; CD272
UniProt ID: (Human) Q7Z6A9
Entrez Gene ID: (Human) 151888
negative regulation of T cell cytokine production
immune response-regulating cell surface receptor signaling pathway
regulation of T cell tolerance induction to tumor cell
regulation of B cell proliferation
negative regulation of T cell proliferation
negative regulation of T cell activation
regulation of ERK1 and ERK2 cascade
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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