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Invitrogen

IL-12/IL-23 p40 Monoclonal Antibody (C8.6), Functional Grade, eBioscience™

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View all (42) IL-12/IL-23 p40 antibodies

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Product Details

16-7129-81

Applications
Tested Dilution
Publications

ELISA (ELISA)

Assay-Dependent
View 1 publication 1 publication

Neutralization (Neu)

Assay-Dependent
View 1 publication 1 publication

Functional Assay (Functional)

Assay-Dependent
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

C8.6

Conjugate

Functional Grade Functional Grade Functional Grade

Form

Liquid

Concentration

1 mg/mL

Amount

50 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_469234

Product Specific Information

Description: The C8.6 antibody reacts with the p40 subunit of human IL-12 p70 and human IL-23. IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and secreted primarily by activated monocytes, macrophages, and dendritic cells. IL-23 is a biologically-related, heterodimeric cytokine composed of disulfide-linked p40 and p19 subunits.

Applications Reported:The C8.6 antibody has been reported for use ELISA, ELISPOT and neutralization.

Applications Tested: The Functional Grade Purified C8.6 antibody has been tested by LAL assay to verify low endotoxin levels and has been tested for ELISA detection and in bioassay for neutralization of IL-12 p70 and IL-23 bioactivity.

The C8.6 antibody at 60.0 ng/mL has been found to inhibit by 50% the biological effects of 1 ng/mL human IL-12 p70 (14-8129) or human IL-23 (14-8239), in corresponding bioassay. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols® section.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level:Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation:Less than 10%, as determined by HPLC.

Target Information

IL-12 is an interleukin produced by dendritic cells, neutrophils, macrophages and human lymphoblastoid cells as a result of antigenic stimulation. IL-12 is a heterodimeric cytokine encoded by IL-12A (p35) and IL-12B (p40). IL-23 is a heterodimeric cytokine that shares a subunit with IL-12 (IL-12B) along with an IL-23A (IL-23p19) subunit. Functionally, IL-12 increases the expression of vascular endothelial adhesion molecule-1 on the endothelium, directs leukocytes towards tumors and facilitates the ischemic-hemorrhagic necrosis of the tumor tissue. In comparison, IL-23 plays an important role in stimulating memory T-cells, and is required for the induction, expansion, maintenance and downstream effector functions of Th17 cells, which play a vital role in upregulating neutrophil chemokines and various pro-inflammatory cytokines.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CLMF p35; CLMF p40; cytotoxic lymphocyte maturation factor 1, p35; Cytotoxic lymphocyte maturation factor 35 kDa subunit; Cytotoxic lymphocyte maturation factor 40 kDa subunit; il 12; IL 12/IL 23p40; il 23; IL 23 p40; IL-12 subunit p35; IL-12 subunit p40; IL-12, subunit p35; IL-12A; IL-12B; IL-23 subunit alpha; IL-23-A; IL-23p19; IL12; IL12 p40; IL23; IL12, subunit p40; il23; IL23 p41; IL35 subunit; ILN; Interleukin; Interleukin 12; interleukin 12, p35; interleukin 12, p40; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); interleukin 23 p19 subunit; interleukin 23, alpha subunit p19; interleukin-12 alpha chain; interleukin-12 beta chain; Interleukin-12 subunit alpha; Interleukin-12 subunit beta; Interleukin-23 subunit alpha; Interleukin-23 subunit p19; interleukin-six, G-CSF related factor; Interleukin12; JKA3 induced upon T-cell activation; natural killer cell stimulatory factor 2; cytotoxic lymphocyte maturation factor 2; IL12p40; natural killer cell stimulatory factor, 40 kD subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; NK cell stimulatory factor chain 2; NKSF/CLMF; IL12p35; 35kD; NKSF/CLMF; IL12p40; 40kD; NKSF1; NKSF2; RP23-388G23.1

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Gene Aliases: CLMF; CLMF2; IL-12A; IL-12B; IL-23; IL-23A; IL12A; IL12B; IL23A; IL23P19; IMD28; IMD29; NFSK; NKSF; NKSF1; NKSF2; P19; P35; SGRF; UNQ2498/PRO5798

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UniProt ID: (Human) P29459, (Human) P29460, (Human) Q9NPF7

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Entrez Gene ID: (Human) 3592, (Human) 3593, (Human) 51561

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Function(s)
cytokine activity interleukin-12 receptor binding protein binding growth factor activity interleukin-12 beta subunit binding interleukin-27 binding protein heterodimerization activity cytokine receptor activity cytokine receptor binding interleukin-12 alpha subunit binding identical protein binding macromolecular complex binding interleukin-23 receptor binding
Process(es)
regulation of cytokine production positive regulation of leukocyte mediated cytotoxicity positive regulation of T cell mediated cytotoxicity positive regulation of defense response to virus by host natural killer cell activation involved in immune response positive regulation of lymphocyte mediated immunity positive regulation of T-helper 1 type immune response positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target negative regulation of inflammatory response to antigenic stimulus cell surface receptor signaling pathway JAK-STAT cascade cell proliferation response to UV-B cell migration natural killer cell activation negative regulation of interleukin-10 production negative regulation of interleukin-17 production positive regulation of granulocyte macrophage colony-stimulating factor production positive regulation of interferon-gamma production positive regulation of interleukin-10 production positive regulation of interleukin-12 production positive regulation of interleukin-17 production positive regulation of tumor necrosis factor production positive regulation of natural killer cell activation positive regulation of natural killer cell proliferation positive regulation of mononuclear cell proliferation positive regulation of tissue remodeling positive regulation of smooth muscle cell apoptotic process interleukin-12-mediated signaling pathway T-helper 1 type immune response T-helper cell differentiation T cell proliferation positive regulation of T cell proliferation positive regulation of activated T cell proliferation T cell activation defense response to protozoan positive regulation of memory T cell differentiation T-helper 1 cell differentiation positive regulation of osteoclast differentiation positive regulation of cell adhesion oocyte development negative regulation of smooth muscle cell proliferation positive regulation of lymphocyte proliferation negative regulation of protein secretion regulation of inflammatory response positive regulation of inflammatory response defense response to Gram-negative bacterium positive regulation of NK T cell activation positive regulation of NK T cell proliferation defense response to virus cellular response to lipopolysaccharide cellular response to interferon-gamma negative regulation of vascular endothelial growth factor signaling pathway positive regulation of NIK/NF-kappaB signaling negative regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis positive regulation of T-helper 17 type immune response positive regulation of T-helper 17 cell lineage commitment immune system process inflammatory response immune response regulation of gene expression positive regulation of macromolecule biosynthetic process innate immune response positive regulation of transcription from RNA polymerase II promoter positive regulation of alpha-beta T cell activation tissue remodeling regulation of primary metabolic process positive regulation of neutrophil chemotaxis receptor signaling pathway via STAT positive regulation of leukocyte differentiation response to virus response to lipopolysaccharide positive regulation of tyrosine phosphorylation of STAT protein positive regulation of natural killer cell mediated cytotoxicity defense response to Gram-positive bacterium extrinsic apoptotic signaling pathway cellular response to virus positive regulation of dendritic cell chemotaxis
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