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Invitrogen
IL-12/IL-23 p40 Monoclonal Antibody (C8.6), Functional Grade, eBioscience™
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Product Details
16-7129-81
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
C8.6
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
50 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The C8.6 antibody reacts with the p40 subunit of human IL-12 p70 and human IL-23. IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and secreted primarily by activated monocytes, macrophages, and dendritic cells. IL-23 is a biologically-related, heterodimeric cytokine composed of disulfide-linked p40 and p19 subunits.
Applications Reported:The C8.6 antibody has been reported for use ELISA, ELISPOT and neutralization.
Applications Tested: The Functional Grade Purified C8.6 antibody has been tested by LAL assay to verify low endotoxin levels and has been tested for ELISA detection and in bioassay for neutralization of IL-12 p70 and IL-23 bioactivity.
The C8.6 antibody at 60.0 ng/mL has been found to inhibit by 50% the biological effects of 1 ng/mL human IL-12 p70 (14-8129) or human IL-23 (14-8239), in corresponding bioassay. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols® section.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level:Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation:Less than 10%, as determined by HPLC.
Target Information
IL-12 is an interleukin produced by dendritic cells, neutrophils, macrophages and human lymphoblastoid cells as a result of antigenic stimulation. IL-12 is a heterodimeric cytokine encoded by IL-12A (p35) and IL-12B (p40). IL-23 is a heterodimeric cytokine that shares a subunit with IL-12 (IL-12B) along with an IL-23A (IL-23p19) subunit. Functionally, IL-12 increases the expression of vascular endothelial adhesion molecule-1 on the endothelium, directs leukocytes towards tumors and facilitates the ischemic-hemorrhagic necrosis of the tumor tissue. In comparison, IL-23 plays an important role in stimulating memory T-cells, and is required for the induction, expansion, maintenance and downstream effector functions of Th17 cells, which play a vital role in upregulating neutrophil chemokines and various pro-inflammatory cytokines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CLMF p35; CLMF p40; cytotoxic lymphocyte maturation factor 1, p35; Cytotoxic lymphocyte maturation factor 35 kDa subunit; Cytotoxic lymphocyte maturation factor 40 kDa subunit; il 12; IL 12/IL 23p40; il 23; IL 23 p40; IL-12 subunit p35; IL-12 subunit p40; IL-12, subunit p35; IL-12A; IL-12B; IL-23 subunit alpha; IL-23-A; IL-23p19; IL12; IL12 p40; IL23; IL12, subunit p40; il23; IL23 p41; IL35 subunit; ILN; Interleukin; Interleukin 12; interleukin 12, p35; interleukin 12, p40; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); interleukin 23 p19 subunit; interleukin 23, alpha subunit p19; interleukin-12 alpha chain; interleukin-12 beta chain; Interleukin-12 subunit alpha; Interleukin-12 subunit beta; Interleukin-23 subunit alpha; Interleukin-23 subunit p19; interleukin-six, G-CSF related factor; Interleukin12; JKA3 induced upon T-cell activation; natural killer cell stimulatory factor 2; cytotoxic lymphocyte maturation factor 2; IL12p40; natural killer cell stimulatory factor, 40 kD subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; NK cell stimulatory factor chain 2; NKSF/CLMF; IL12p35; 35kD; NKSF/CLMF; IL12p40; 40kD; NKSF1; NKSF2; RP23-388G23.1
Gene Aliases: CLMF; CLMF2; IL-12A; IL-12B; IL-23; IL-23A; IL12A; IL12B; IL23A; IL23P19; IMD28; IMD29; NFSK; NKSF; NKSF1; NKSF2; P19; P35; SGRF; UNQ2498/PRO5798
UniProt ID: (Human) P29459, (Human) P29460, (Human) Q9NPF7
Entrez Gene ID: (Human) 3592, (Human) 3593, (Human) 51561
cytokine activity
interleukin-12 receptor binding
protein binding
growth factor activity
interleukin-12 beta subunit binding
interleukin-27 binding
protein heterodimerization activity
cytokine receptor activity
cytokine receptor binding
interleukin-12 alpha subunit binding
identical protein binding
macromolecular complex binding
interleukin-23 receptor binding
regulation of cytokine production
positive regulation of leukocyte mediated cytotoxicity
positive regulation of T cell mediated cytotoxicity
positive regulation of defense response to virus by host
natural killer cell activation involved in immune response
positive regulation of lymphocyte mediated immunity
positive regulation of T-helper 1 type immune response
positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target
negative regulation of inflammatory response to antigenic stimulus
cell surface receptor signaling pathway
JAK-STAT cascade
cell proliferation
response to UV-B
cell migration
natural killer cell activation
negative regulation of interleukin-10 production
negative regulation of interleukin-17 production
positive regulation of granulocyte macrophage colony-stimulating factor production
positive regulation of interferon-gamma production
positive regulation of interleukin-10 production
positive regulation of interleukin-12 production
positive regulation of interleukin-17 production
positive regulation of tumor necrosis factor production
positive regulation of natural killer cell activation
positive regulation of natural killer cell proliferation
positive regulation of mononuclear cell proliferation
positive regulation of tissue remodeling
positive regulation of smooth muscle cell apoptotic process
interleukin-12-mediated signaling pathway
T-helper 1 type immune response
T-helper cell differentiation
T cell proliferation
positive regulation of T cell proliferation
positive regulation of activated T cell proliferation
T cell activation
defense response to protozoan
positive regulation of memory T cell differentiation
T-helper 1 cell differentiation
positive regulation of osteoclast differentiation
positive regulation of cell adhesion
oocyte development
negative regulation of smooth muscle cell proliferation
positive regulation of lymphocyte proliferation
negative regulation of protein secretion
regulation of inflammatory response
positive regulation of inflammatory response
defense response to Gram-negative bacterium
positive regulation of NK T cell activation
positive regulation of NK T cell proliferation
defense response to virus
cellular response to lipopolysaccharide
cellular response to interferon-gamma
negative regulation of vascular endothelial growth factor signaling pathway
positive regulation of NIK/NF-kappaB signaling
negative regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis
positive regulation of T-helper 17 type immune response
positive regulation of T-helper 17 cell lineage commitment
immune system process
inflammatory response
immune response
regulation of gene expression
positive regulation of macromolecule biosynthetic process
innate immune response
positive regulation of transcription from RNA polymerase II promoter
positive regulation of alpha-beta T cell activation
tissue remodeling
regulation of primary metabolic process
positive regulation of neutrophil chemotaxis
receptor signaling pathway via STAT
positive regulation of leukocyte differentiation
response to virus
response to lipopolysaccharide
positive regulation of tyrosine phosphorylation of STAT protein
positive regulation of natural killer cell mediated cytotoxicity
defense response to Gram-positive bacterium
extrinsic apoptotic signaling pathway
cellular response to virus
positive regulation of dendritic cell chemotaxis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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