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Invitrogen
IFN gamma Monoclonal Antibody (XMG1.2), Functional Grade, eBioscience™
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FIGURE: 1 / 96
IFN gamma Antibody (16-7311-81) in WB, IHC
Autophagy 2014 -
Figure 1. MSCs undergo autophagy in the inflammatory microenvironment. ( A and B ) MAP1LC3-EGFP-MSCs were injected into naive and EAE (on day 15) mice via tail vein. Mice were euthanized at 24, 48, 72, and 120 h later, the lungs and spinal cords were separated and cut into frozen sections. Sections were stained with DAPI and examined by confocal microscopy. ( C ) Immunoblot analysis of MSCs starved for 0 (c... View More
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
.jpg?time=20220826?time=20220826 "IFN gamma Antibody (16-7311-81) in Flow")
Product Details
16-7311-81
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Human,
Mouse,
Rat
Host/Isotype
Rat
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
XMG1.2
Conjugate
Functional Grade
Functional Grade
Functional Grade
Form
Liquid
Concentration
1 mg/mL
Amount
50 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The XMG1.2 antibody reacts with mouse interferon (IFN) gamma. The XMG1.2 antibody is a neutralizing antibody. Mouse IFN gamma is a 20 kDa factor produced by activated T, B and NK cells, and is an anti-viral and anti-parasitic cytokine. IFN gamma, in synergy with other cytokines such as TNF alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN gamma. Glycosylation of IFN gamma does not affect its biological activity.
Applications Tested: The Functional Grade Purified XMG1.2 antibody has been tested by LAL assay to verify low endotoxin levels and has been tested for ELISA capture and in bioassay for neutralization of IFN gamma bioactivity.
The XMG1.2 antibody at 15 ng/mL has been found to inhibit by 50% the biological effects of 1 ng/mL of mouse IFN gamma (ND50), in an EMCV assay of L929 cell protection. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols® section.
The XMG1.2 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse IFN gamma in combination with the biotin R4-6A2 (13-7312) antibody for detection and recombinant mouse IFN gamma (39-8311) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 0.25 - 1 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level:Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation:Less than 10%, as determined by HPLC.
Target Information
IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cytokine; gamma interferon; H-IFN-g; IFN γ; IFN-gamma; IFN-y; IFNγ; Interferon; interferon 2f; interferon gamma precursor; Interferon y; Interferon γ; Interferonγ; M-IFN-g; R-IFN-g; unnamed protein product
Gene Aliases: If2f; Ifg; IFN-g
UniProt ID: (Mouse) P01580
Entrez Gene ID: (Mouse) 15978
negative regulation of transcription from RNA polymerase II promoter
microglial cell activation
neutrophil apoptotic process
positive regulation of cytokine production
regulation of the force of heart contraction
adaptive immune response
macrophage activation involved in immune response
CD8-positive, alpha-beta T cell differentiation involved in immune response
positive regulation of T cell cytokine production
regulation of transcription, DNA-templated
apoptotic process
inflammatory cell apoptotic process
immune response
humoral immune response
JAK-STAT cascade
positive regulation of cell proliferation
negative regulation of cell proliferation
response to virus
positive regulation of autophagy
positive regulation of gene expression
negative regulation of gene expression
positive regulation of epithelial cell migration
urea metabolic process
antigen processing and presentation
macrophage differentiation
neutrophil chemotaxis
negative regulation of epithelial cell differentiation
endoplasmic reticulum unfolded protein response
positive regulation of protein complex assembly
negative regulation of myelination
regulation of neurological system process
positive regulation of synaptic transmission, cholinergic
negative regulation of interleukin-17 production
positive regulation of chemokine production
positive regulation of interleukin-1 beta production
positive regulation of interleukin-12 production
positive regulation of interleukin-23 production
positive regulation of interleukin-6 production
positive regulation of tumor necrosis factor production
positive regulation of peptidyl-serine phosphorylation of STAT protein
positive regulation of smooth muscle cell apoptotic process
Fc-gamma receptor signaling pathway involved in phagocytosis
interleukin-2-mediated signaling pathway
type III interferon-mediated signaling pathway
positive regulation of T cell proliferation
macrophage activation
positive regulation of protein import into nucleus
positive regulation of tyrosine phosphorylation of STAT protein
defense response to bacterium
defense response to protozoan
positive regulation of apoptotic process
positive regulation by host of viral process
positive regulation of MHC class II biosynthetic process
positive regulation of nitric oxide biosynthetic process
positive regulation of neuron differentiation
negative regulation of osteoclast differentiation
positive regulation of osteoclast differentiation
positive regulation of cell adhesion
positive regulation of glycolytic process
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
creatinine metabolic process
astrocyte activation
negative regulation of fibroblast proliferation
positive regulation of isotype switching to IgG isotypes
negative regulation of smooth muscle cell proliferation
regulation of defense response to virus by host
positive regulation of inflammatory response
positive regulation of phagocytosis
positive regulation of neurogenesis
regulation of immune response
regulation of insulin secretion
defense response to Gram-positive bacterium
T cell receptor signaling pathway
sensory perception of mechanical stimulus
positive regulation of membrane protein ectodomain proteolysis
defense response to virus
regulation of glial cell proliferation
interferon-gamma-mediated signaling pathway
positive regulation of fructose 1,6-bisphosphate metabolic process
positive regulation of vitamin D biosynthetic process
cellular response to lipopolysaccharide
cellular response to interleukin-18
negative regulation of glomerular mesangial cell proliferation
extrinsic apoptotic signaling pathway
receptor signaling pathway via STAT
regulation of neuronal action potential
neuroinflammatory response
negative regulation of beta-amyloid clearance
positive regulation of glutamate receptor signaling pathway
positive regulation of cellular respiration
positive regulation of beta-amyloid formation
response to sodium arsenite
positive regulation of iron ion import across plasma membrane
positive regulation of STAT cascade
positive regulation of tumor necrosis factor (ligand) superfamily member 11 production
regulation of hepatocyte proliferation
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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