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CD130 (GP130) Monoclonal Antibody (KGP130), APC, eBioscience™
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FIGURE: 1 / 2
CD130 (GP130) Antibody (17-1302-82) in Flow
Staining of C57Bl/6 splenocytes with Anti-Mouse CD3e FITC (Product # 11-0031-82) and 0.125 µg of Rat IgG2a K Isotype Control APC (Product # 17-4321-81) (left) or 0.125 µg of Anti-Mouse CD130 APC (right). Cells in the lymphocyte gate were used for analysis.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
17-1302-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
KGP130
Conjugate
APC
APC
APC
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The KGP130 monoclonal antibody reacts with mouse gp130, also known as CD130. gp130 is a 130 kD Type-I transmembrane glycoprotein containing a 597 amino acid extracellular domain, a single transmembrane domain, and a 277 amino acid cytoplasmic domain. gp130 is a subunit of several heterodimeric cell-surface receptors, including receptors for IL-6, IL-11, IL-27, Oncostatin M, and Leukemia Inhibitory Factor (LIF). The gp130 protein has also been found to exist in a soluble form, which is capable of inhibiting IL-6 signaling. gp130 is expressed mainly on T cells, monocytes, endothelial cells, activated B cells, and plasma cells, and is expressed at lower levels on most leukocytes and epithelial cells. In response to ligand binding, gp130 becomes tyrosine phosphorylated, leading to activation of several signaling pathways including the PI3 kinase, Ras-MAPk and Stat pathways.
Preincubation of mouse splenic T cells with IL-6 is capable of blocking the interaction of KGP130 with gp130, suggesting that the KGP130 binding site lies near the IL-6 interaction domain. Reactivity of KGP130 towards human gp130 has not been observed.
Applications Reported: This KGP130 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This KGP130 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The protein encoded by this gene is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggested a critical role of the gene encoding this protein in regulating myocyte apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GP130-RAPS; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6R-beta; IL-6RB; IL6R-beta; membrane glycoprotein 130; oncostatin-M receptor subunit alpha; unnamed protein product
Gene Aliases: 5133400A03Rik; CD130; D13Ertd699e; gp130
UniProt ID: (Mouse) Q00560
Entrez Gene ID: (Mouse) 16195
cytokine receptor activity
ciliary neurotrophic factor receptor activity
interleukin-6 receptor activity
interleukin-11 receptor activity
leukemia inhibitory factor receptor activity
oncostatin-M receptor activity
cytokine activity
ciliary neurotrophic factor receptor binding
interleukin-6 receptor binding
protein binding
coreceptor activity
growth factor binding
cytokine binding
interleukin-11 binding
interleukin-6 binding
protein tyrosine kinase activator activity
identical protein binding
interleukin-27 receptor activity
scaffold protein binding
columnar/cuboidal epithelial cell differentiation
positive regulation of adaptive immune response
glycogen metabolic process
triglyceride mobilization
signal transduction
JAK-STAT cascade
cell proliferation
positive regulation of cell proliferation
regulation of Notch signaling pathway
positive regulation of smooth muscle cell migration
cytokine-mediated signaling pathway
cell differentiation
response to cytokine
autocrine signaling
interleukin-11-mediated signaling pathway
oncostatin-M-mediated signaling pathway
positive regulation of T cell proliferation
negative regulation of neuron apoptotic process
positive regulation of osteoblast differentiation
positive regulation of Notch signaling pathway
positive regulation of astrocyte differentiation
leukemia inhibitory factor signaling pathway
negative regulation of cytosolic calcium ion concentration
intestinal epithelial cell development
interleukin-6-mediated signaling pathway
negative regulation of interleukin-6-mediated signaling pathway
interleukin-27-mediated signaling pathway
ciliary neurotrophic factor-mediated signaling pathway
receptor signaling pathway via STAT
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