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Invitrogen
CD244.1 Monoclonal Antibody (C9.1), APC, eBioscience™
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FIGURE: 1 / 1
CD244.1 Antibody (17-2440-82) in Flow
Staining of BALB/c splenocytes with Anti-Mouse CD49b (Integrin alpha 2) FITC (Product # 11-5971-82) and 0.125 µg of Rat IgG2b K Isotype Control APC (Product # 17-4031-82) (left) or 0.125 µg of Anti-Mouse CD244-1 APC (right). Total cells were used for analysis.
Product Details
17-2440-82
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
C9.1
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The monoclonal antibody C9.1 reacts with mouse CD244.1. This receptor is expressed on NK cells, gamma delta T cells, some CD8+ T cells, basophils, and monocytes on most inbred mouse strains except C57BL/6 and C58, which express the CD244.2 allotype. CD244 is a CD2-related receptor belonging to the SLAM family that exists as two different isoforms with opposing functions. CD244 associates with CD48, which is expressed widely on hematopoietic cells, regulates NK cell proliferation, cytotoxicity, and cytokine production. However, the CD244/CD28 interaction between NK and other cell types inhibits NK cytotoxicity, which contributes to self-tolerance. CD244 may enhance or inhibit CD8+ T cell effector function depending on SAP levels.
The C9.1 antibody has been reported to have antagonist activity on activated NK cells.
Applications Reported: This C9.1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This C9.1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
This gene encodes a cell surface receptor expressed on natural killer (NK) cells (and some T cells) that mediate non-major histocompatibility complex (MHC) restricted killing. The interaction between NK-cell and target cells via this receptor is thought to modulate NK-cell cytolytic activity. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD244; CD244 long isoform; CD244 short isoform; natural killer cell receptor; Natural killer cell receptor 2B4; NK cell receptor 2B4; NK cell type I receptor protein 2B4; NKR2B4; non MHC restricted killing associated; Non-MHC restricted killing associated; Signaling lymphocytic activation molecule 4; SLAM family member 4; SLAMF4
Gene Aliases: 2B4; C9.1; Cd244; Cd244a; F730046O15Rik; Ly90; NAIL; NKR2B4; Nmrk; SLAMF4
UniProt ID: (Mouse) Q07763
Entrez Gene ID: (Mouse) 18106
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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