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Invitrogen
CD218a (IL-18Ra) Monoclonal Antibody (H44), APC, eBioscience™
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FIGURE: 1 / 1
CD218a (IL-18Ra) Antibody (17-7183-42) in Flow
Staining of normal human peripheral blood cells with Anti-Human CD3 PerCP-eFluor® 710 (Product # 46-0036-42) and Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (left) or Anti-Human CD218a (IL-18 Receptor alpha) APC (right). Cells in the lymphocyte gate were used for analysis.
Product Details
17-7183-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
H44
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The H44 antibody reacts with the interleukin-18 receptor alpha chain, also known as IL-1Rrp, an IL-1R family member. IL-18R alpha is expressed on Th1 cells, NK cell subset, neutrophils and IL-12-stimulated tonsillar B cells. By itself, IL-18R alpha binds IL-18 with low affinity and when complexed with IL-18R beta chain binds IL-18 with high affinity. The proinflammatory cytokine IL-18 and its receptor complex play an important role in the innate and autoimmune responses.
Applications Reported: This H44 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This H44 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
It is recommended to stain whole blood before RBC lysis.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
IL18R1 is a cytokine receptor that belongs to the interleukin 1 receptor family. This receptor specifically binds interleukin 18 (IL18), and is essential for IL18 mediated signal transduction. IFN-alpha and IL12 are reported to induce the expression of this receptor in NK and T cells. The gene along with four other members of the interleukin 1 receptor family, including IL1R2, IL1R1, ILRL2 (IL-1Rrp2), and IL1RL1 (T1/ST2), form a gene cluster on chromosome 2q.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD218 antigen-like family member A; CD218a; CDw218a; cytokine receptor; IL-18R-1; IL-18R-alpha; IL-1Rrp; IL1 receptor-related protein; Interleukin-18; Interleukin-18 receptor 1; Interleukin-18 receptor alpha
Gene Aliases: CD218a; CDw218a; IL-18R-alpha; IL-18Ralpha; IL-1Rrp; IL18R1; IL18RA; IL18Ralpha2; IL1RRP
UniProt ID: (Human) Q13478
Entrez Gene ID: (Human) 8809
adaptive immune response
inflammatory response
immune response
signal transduction
natural killer cell activation
positive regulation of interferon-gamma production
interleukin-18-mediated signaling pathway
T-helper 1 cell differentiation
positive regulation of NF-kappaB transcription factor activity
negative regulation of cold-induced thermogenesis
positive regulation of NIK/NF-kappaB signaling
positive regulation of T-helper 1 cell cytokine production
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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