Search

  • Contact Us
  • Quick Order

Invitrogen

HIF-1 alpha Monoclonal Antibody (Mgc3), APC, eBioscience™

Advanced Verification
2 Published Figures
3 References
View all (89) HIF1A antibodies

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Additional Information:
{{banner.description}}
{{$ctrl.isSkuNotesCollapsed ? 'View more' : 'View less'}}
HIF-1 alpha Monoclonal Antibody (Mgc3), APC, eBioscience™

Product Details

17-7528-82

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

1.0 µg/test
View 3 publications 3 publications
Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

Mgc3

Immunogen

Human HIF-1 alpha amino acids 530-826.
3D Epitope / Immunogen

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra spectra

Form

Liquid

Concentration

0.2 mg/mL

Amount

100 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2802231

Product Specific Information

Description: This Mgc3 monoclonal antibody detects hypoxia-inducible factor 1 alpha (HIF-1 alpha) from human, non-human primate, bovine, mouse and porcine cells. This antibody does not cross-react with ARNT or the related HIF-2 alpha.

Mgc3 has been successfully used in western blotting, immunofluorescence, immunocytochemistry, immunoprecipitation, flow cytometry and gel shift procedures. By western blot, this antibody detects a ~93 kDa protein representing HIF-1 alpha after hypoxic induction in COS cells. Immunofluorescence staining of HIF-1 alpha in COS-7 cells with clone Mgc3 yields nuclear staining after exposing cells to 1% oxygen for 4 hours. In gel shift assay experiments, the Mgc3 antibody detected only mouse and not human HIF-1 alpha.

Other anti-HIF1 alpha antibodies were previously included in a Thermo Scientific Cellomics High Content Screening Kit. Clone Mgc3 is now recommended as a replacement. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. High content assays may require further optimization, including the selection of the optimal Dylight-conjugated secondary antibody.

Applications Reported: This Mgc3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This Mgc3 antibody has been tested by flow cytometric analysis of DFOA stimulated HeLa cells using the methanol fixation protocol. This may be used at less than or equal to 1.0 µg/mL per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser

Target Information

HIF1-alpha (HIF1A) is a subunit of HIF1, which is a transcription factor found in mammalian cells cultured under reduced oxygen tension. HIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors. HIF1 functions as a transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, HIF-1 activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1-alpha regulates hypoxia-mediated apoptosis, cell proliferation and tumor angiogenesis. Hypoxia which induces p53 protein accumulation, directly interacts with HIF1-alpha and reduces hypoxia-induced expression of HIF1-alpha by promoting MDM2-mediated ubiquitination and proteasomal degradation under hypoxic conditions. Recent studies suggest that induction of NOX4 by HIF1-alpha contributes to maintain ROS levels after hypoxia and hypoxia-induced proliferation. In humans, it is located on the q arm of chromosome 14. The C-terminal of HIF1A binds to p300. p300/CBP-HIF complexes participate in the induction of hypoxia-responsive genes, including VEGF. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Bioinformatics

Protein Aliases: ARNT interacting protein; ARNT-interacting protein; ARNT2; Basic-helix-loop-helix-PAS protein MOP1; bHLHe78; Class E basic helix-loop-helix protein 78; hif 1; hif 1a; HIF-1-alpha; HIF-1alpha 786; HIF1-alpha; hypoxia inducible factor 1 alpha subunit; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); Hypoxia-inducible factor 1-alpha; hypoxia-inducible factor1alpha; Member of PAS protein 1; member of PAS superfamily 1; PAS domain-containing protein 8; transcription factor; transcription factor; hypoxia-inducible factor 1a; unnamed protein product

View more View less

Gene Aliases: AA959795; BHLHE78; HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; HIF1A; HIF1alpha; MOP1; PASD8

View more View less

UniProt ID: (Human) Q16665, (Mouse) Q61221

View more View less

Entrez Gene ID: (Human) 3091, (Mouse) 15251

View more View less

Function(s)
RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding bacterial-type RNA polymerase transcriptional activator activity, sequence-specific DNA binding bacterial-type RNA polymerase transcriptional repressor activity, sequence-specific DNA binding transcription corepressor binding transcription coactivator binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding p53 binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding ligand-dependent nuclear receptor binding enzyme binding protein kinase binding protein domain specific binding ubiquitin protein ligase binding histone deacetylase binding sequence-specific DNA binding protein heterodimerization activity protein dimerization activity Hsp90 protein binding RNA polymerase II sequence-specific DNA binding transcription factor binding E-box binding transcription regulator activator activity transcription factor activity, transcription factor binding transcription factor activity, RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding signal transducer activity transcription factor binding protein complex binding histone acetyltransferase binding nuclear hormone receptor binding basic helix-loop-helix transcription factor
Process(es)
response to reactive oxygen species angiogenesis blood vessel development response to hypoxia cellular glucose homeostasis neural crest cell migration epithelial to mesenchymal transition embryonic placenta development B-1 B cell homeostasis positive regulation of endothelial cell proliferation vasculature development heart looping positive regulation of neuroblast proliferation chondrocyte differentiation glandular epithelial cell differentiation glandular epithelial cell maturation connective tissue replacement involved in inflammatory response wound healing outflow tract morphogenesis cardiac ventricle morphogenesis lactate metabolic process regulation of glycolytic process regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter cellular iron ion homeostasis apoptotic process signal transduction neuroblast proliferation lactation visual learning response to iron ion regulation of gene expression vascular endothelial growth factor production positive regulation of vascular endothelial growth factor production positive regulation of gene expression negative regulation of gene expression positive regulation of epithelial cell migration response to muscle activity positive regulation of macroautophagy axonal transport of mitochondrion neural fold elevation formation cerebral cortex development cell differentiation negative regulation of ossification bone mineralization negative regulation of bone mineralization positive regulation of vascular endothelial growth factor receptor signaling pathway TOR signaling negative regulation of TOR signaling oxygen homeostasis positive regulation of chemokine production regulation of transforming growth factor beta2 production collagen metabolic process cellular response to oxidative stress embryonic hemopoiesis insulin secretion involved in cellular response to glucose stimulus positive regulation of insulin secretion involved in cellular response to glucose stimulus regulation of cell proliferation hemoglobin biosynthetic process glucose homeostasis negative regulation of apoptotic process negative regulation of neuron apoptotic process positive regulation of blood vessel endothelial cell migration positive regulation of erythrocyte differentiation positive regulation of angiogenesis positive regulation of glycolytic process positive regulation of transcription, DNA-templated negative regulation of growth positive regulation of transcription from RNA polymerase II promoter muscle cell cellular homeostasis positive regulation of hormone biosynthetic process blood vessel morphogenesis digestive tract morphogenesis camera-type eye morphogenesis cartilage development neuron apoptotic process elastin metabolic process intestinal epithelial cell maturation epithelial cell differentiation involved in mammary gland alveolus development iris morphogenesis retina vasculature development in camera-type eye positive regulation of chemokine-mediated signaling pathway regulation of thymocyte apoptotic process negative regulation of thymocyte apoptotic process cellular response to interleukin-1 cellular response to hypoxia dopaminergic neuron differentiation mesenchymal cell apoptotic process hypoxia-inducible factor-1alpha signaling pathway cellular response to virus positive regulation of cytokine production involved in inflammatory response negative regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway positive regulation of nitric oxide metabolic process regulation of protein neddylation negative regulation of mesenchymal cell apoptotic process transcription, DNA-templated transcription from RNA polymerase II promoter positive regulation of cell proliferation positive regulation of autophagy positive regulation of receptor biosynthetic process negative regulation of transcription elongation from RNA polymerase II promoter positive regulation of apoptotic process regulation of transcription from RNA polymerase II promoter in response to oxidative stress negative regulation of vasoconstriction positive regulation of smooth muscle cell proliferation regulation of catalytic activity response to fungicide positive regulation of transcription from RNA polymerase II promoter in response to hypoxia positive regulation of mitophagy regulation of aerobic respiration negative regulation of reactive oxygen species metabolic process
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support