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Invitrogen
CD155 Monoclonal Antibody (2H7CD155), PE-Cyanine7, eBioscience™
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FIGURE: 1 / 1
CD155 Antibody (25-1550-42) in Flow
U937 cells were stained with Mouse IgG1 kappa Isotype Control, PE-Cyanine7 (Product # 25-4714-80) (blue histogram) or CD155 Monoclonal Antibody, PE-Cyanine7 (purple histogram). Total viable cells were used for analysis, as determined by Fixable Viability Dye eFluor 450 (Product # 65-0863-18).
Product Details
25-1550-42
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
2H7CD155
Conjugate
Excitation/Emission Max
569/780 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The monoclonal antibody 2H7CD155 recognizes CD155 also known as the poliovirus receptor. The CD155 protein is a member of the Ig superfamily in which 4 isoforms exist; only the alpha and delta forms are expressed on the membrane of monocytes, prostate cancer cells, as well as neurectodermal and monocytic tumor cells lines. In addition, CD155 is found during mid-gastrulation during embryonic development. CD155 is thought to play a role in adhesion by interaction with the ECM component vitronectin as well as a role in NK killing of tumor cells. CD226 and CD96 are ligands for CD155. Blocking of CD155 is under therapeutic investigation for prevention of variants of poliovirus.
Applications Reported: This 2H7CD155 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 2H7CD155 antibody has been pre-diluted and tested by flow cytometric analysis of U937 cell line. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Target Information
The protein encoded by this gene is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. The external domain mediates cell attachment to the extracellular matrix molecule vitronectin, while its intracellular domain interacts with the dynein light chain Tctex-1/DYNLT1. The gene is specific to the primate lineage, and serves as a cellular receptor for poliovirus in the first step of poliovirus replication. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD155; FLJ25946; NECL-5; nectin-like 5; Nectin-like protein 5; Poliovirus receptor; sCD112; soluble CD112; unnamed protein product
Gene Aliases: CD155; HVED; Necl-5; NECL5; PVR; PVS; TAGE4
UniProt ID: (Human) P15151
Entrez Gene ID: (Human) 5817
positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target
cell adhesion
homophilic cell adhesion via plasma membrane adhesion molecules
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
signal transduction
natural killer cell mediated cytotoxicity
susceptibility to natural killer cell mediated cytotoxicity
negative regulation of natural killer cell mediated cytotoxicity
positive regulation of natural killer cell mediated cytotoxicity
viral entry into host cell
susceptibility to T cell mediated cytotoxicity
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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