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Invitrogen
beta Tubulin Monoclonal Antibody (2 28 33)
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FIGURE: 1 / 34
beta Tubulin Antibody (32-2600) in ICC/IF
Visualizing a rat cortical neuron culture. Cryopreserved Gibco™ Primary Mouse Cortical Neurons (Product # A15585) were grown in culture for 3 weeks using the Gibco™ B-27™ Plus Neuronal Culture System (Product # A3653401). Cells were fixed and labeled with Invitrogen™ NucBlue™ Fixed Cell ReadyProbes™ Reagent (Product # R37606), anti-β-3 tubulin mouse monoclonal antibody (Product # 32-2600) in conjunction wit... View More
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.jpg?time=20220826?time=20220826 "beta Tubulin Antibody (32-2600) in WB")
Product Details
32-2600
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
C. elegans,
Human,
Mouse,
Non-human primate,
Rat
Published species
C. elegans,
Dog,
Fruit fly,
Human,
Mouse,
Non-human primate,
Pig,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
2 28 33
Immunogen
Beta-tubulin from sea urchin (S. purpuratus) sperm.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody reacts with the ~50 kDa beta-tubulin and has been shown to bind to the two major and one of the minor beta-tubulin isotypes. Reactivity has been confirmed with mouse NIH3T3 fibroblast cells, rat brain, and mouse testis.
Target Information
Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Beta-III tubulin is primarily expressed in neurons and may be involved in neurogenesis, axon guidance and maintenance. Mutations in the beta tubulin gene are the cause of congenital fibrosis of the extraocular muscles type 3. Beta-III tubulin was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues. Glutamate residues at the C-terminus of beta III tubulin can be glutamylated. The precise function of such modifications is unclear. Tubulin is phosphorylated on Ser-172 by CDK1 during cell cycle progression. Ser-172 phosphorylation inhibits tubulin incorporation into microtubules.Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55 kDa subunits: alpha and Beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. Because beta-tubulin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: beta Ib tubulin; beta tubulin 1; beta-4 tubulin; beta-tubulin T beta15 (aa 1-445); betaIII-tubulin; class I beta-tubulin; class II beta-tubulin isotype; class IIa beta-tubulin; class IIb beta-tubulin; class III beta-tubulin; class IVa beta-tubulin; class V beta-tubulin; dJ40E16.7; dystonia 4, torsion (autosomal dominant); HSA10p15 beta-tubulin 4Q; M40 antibody; Neuron-specific class III beta-tubulin; T beta-15; tubb2a {ECO:0000250|UniProtKB:Q7TMM9}; Tubulin 5 beta; tubulin beta; Tubulin beta 8 class VIII; Tubulin beta chain; Tubulin beta class IIa; Tubulin beta class V; tubulin beta MGC4083; Tubulin beta-1 chain; Tubulin beta-2A chain; Tubulin beta-2B chain; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-4A chain; Tubulin beta-5 chain; Tubulin beta-6 chain; Tubulin beta-8 chain; Tubulin beta-III; tubulin, beta 1 class VI; tubulin, beta 2; tubulin, beta 2A class IIa; tubulin, beta 2B class IIb; tubulin, beta 3 class III; tubulin, beta 4; tubulin, beta 4 class IVa; tubulin, beta 4A class IVa; tubulin, beta 6 class V; tubulin, beta class I; tubulin, beta polypeptide; tubulin, beta polypeptide 2; tubulin, beta polypeptide paralog; tubulin, beta, 5
Gene Aliases: 2310057H16Rik; 2410129E14Rik; 2810484G07Rik; 3200002H15Rik; AA408537; AI325297; AI596182; B130022C14Rik; bA506K6.1; bA631M21.2; BB220206; beta-4; beta-5; brdp; CDCBM; CDCBM1; CDCBM5; CDCBM6; CFEOM3; CFEOM3A; CSCSC1; DYT4; FEOM3; HsT1601; M(beta)1; M(beta)2; M(beta)3; M(beta)4; M(beta)5; M(beta)6; M40; OK/SW-cl.56; OOMD; OOMD2; PMGYSA; RGD1305887; RGD1309427; TUBB; TUBB-5; TUBB1; TUBB2; TUBB2A; TUBB2B; TUBB3; TUBB4; TUBB4A; TUBB5; TUBB6; TUBB8
UniProt ID: (Human) P07437, (Human) Q9H4B7, (Human) Q13885, (Human) A8K068, (Human) Q13509, (Human) P04350, (Human) Q9BUF5, (Human) Q3ZCM7, (Mouse) A2AQ07, (Rat) P85108, (Mouse) Q7TMM9, (Mouse) Q9CWF2, (Rat) Q3KRE8, (Mouse) Q9ERD7, (Rat) Q4QRB4, (Mouse) Q9D6F9, (Mouse) P99024, (Rat) P69897, (Mouse) Q922F4
Entrez Gene ID: (Dog) 474830, (Human) 203068, (Human) 81027, (Dog) 485950, (Human) 7280, (Dog) 478702, (Human) 347733, (Dog) 102154194, (Human) 10381, (Dog) 100855815, (Human) 10382, (Human) 84617, (Dog) 480213, (Human) 347688, (Mouse) 545486, (Rat) 679312, (Rat) 498736, (Mouse) 22151, (Mouse) 73710, (Rat) 291081, (Mouse) 22152, (Rat) 246118, (Rat) 29213, (Mouse) 22153, (Mouse) 22154, (Rat) 29214, (Mouse) 67951, (Rat) 307351
GTPase activity
structural molecule activity
structural constituent of cytoskeleton
protein binding
GTP binding
protein domain specific binding
ubiquitin protein ligase binding
protein complex binding
GTPase activating protein binding
MHC class I protein binding
peptide binding
molecular_function
nucleotide binding
tubulin
G2/M transition of mitotic cell cycle
movement of cell or subcellular component
microtubule-based process
cellular process
cytoskeleton-dependent intracellular transport
natural killer cell mediated cytotoxicity
spindle assembly
cell division
cytoskeleton organization
neuron migration
mitotic nuclear division
axon guidance
biological_process
protein polymerization
metabolic process
neuron differentiation
cell projection organization
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