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Invitrogen
PTBP1 Monoclonal Antibody (1)
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FIGURE: 1 / 79
PTBP1 Antibody (32-4800) in ICC/IF
Immunofluorescence analysis of PTB (CLONE 1) was performed using 70% confluent log phase HCT 116 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PTBP1/PTB (1) Mouse Monoclonal Antibody (Product # 32-4800) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Prod... View More
Product Details
32-4800
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse,
Rat,
Virus
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
1
Immunogen
Recombinant human PTB protein.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody reacts with the C-terminus of the human and rodent PTB protein.
Target Information
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has four repeats of quasi-RNA recognition motif (RRM) domains that bind RNAs. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. This protein is localized in the nucleoplasm and it is also detected in the perinucleolar structure. Alternatively spliced transcript variants encoding different isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 57 kDa RNA-binding protein PPTB-1; Heterogeneous nuclear ribonucleoprotein I; heterogeneous nuclear ribonucleoprotein polypeptide I; hnRNP I; MGC10830; MGC8461; polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoprotein I); Polypyrimidine tract-binding protein 1; PTB; PYBP; pyrimidine binding protein 1; pyrimidine binding protein 2; Pyrimidine-binding protein; RNA-binding protein
Gene Aliases: AA407203; AL033359; HNRNP-I; HNRNPI; HNRPI; pPTB; PTB; PTB-1; PTB-T; PTB2; PTB3; PTB4; PTBP1; Pybp; Pybp1; Pybp2; Tbfii
UniProt ID: (Human) P26599, (Mouse) P17225, (Rat) Q00438
Entrez Gene ID: (Human) 5725, (Mouse) 19205, (Rat) 29497
regulation of alternative mRNA splicing, via spliceosome
mRNA processing
RNA splicing
gene expression
neurogenesis
negative regulation of RNA splicing
regulation of RNA splicing
regulation of cell differentiation
negative regulation of neuron differentiation
positive regulation of transcription from RNA polymerase II promoter
negative regulation of mRNA splicing, via spliceosome
negative regulation of muscle cell differentiation
positive regulation of calcineurin-NFAT signaling cascade
IRES-dependent viral translational initiation
negative regulation of mRNA metabolic process
nucleic acid phosphodiester bond hydrolysis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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