Mouse Flt3 Ligand (Flt3L) Carrier-Free Recombinant Protein

Product Specific Information

Description: Flt3 (FMS-related tyrosine kinase 3) Ligand is a growth factor that stimulates the growth and differentiation of early hematopoetic progenitor cells. Flt3 Ligand alone exerts weak biological effects, and instead synergizes with other colony-stimulating factors to induce the proliferation and differentiation of cells of the myeloid and lymphoid lineages. Recent studies have suggested that Flt3 Ligand plays an important role in the development and expansion of dendritic cells in vivo, and that it may influence NK cell survival by this mechanism.

Flt3 Ligand transcripts are detectable in most cell types and tissues, although the protein has only been found in stromal cells and T cells. It is expressed most often as a transmembrane cell surface protein, although processing can yield a soluble, secreted isoform.

Applications Reported: Mouse Flt3 Ligand Recombinant Protein Carrier-Free is biologically active.

Applications Tested: The ED50 of this protein, as measured by IL-6 induction in M1 cells, is 50-250 ng/mL in the presence of 1 ng/mL Mouse LIF Recombinant Protein. This corresponds to a specific activity of 2 x 10e4 - 4 x 10e3 Units/mg.

Source: E. coli expressed amino acids Thr28-Gln189 accession number NM_013520.

Bioactivity: The ED50 of this protein, as measured by IL-6 induction in M1 cells, is 50-250 ng/mL in the presence of 1 ng/mL Mouse LIF Recombinant Protein. This corresponds to a specific activity of 2 x 10e4 - 4 x 10e3 Units/mg.

Endotoxin: Less than 0.01 ng/ug cytokine as determined by the LAL assay. Purity: >97% as determined by SDS-PAGE.

Molecular Weight: 20.7 kDa.

Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment.

Target Information

FLT3LG (Fms Related Tyrosine Kinase 3 Ligand) is a Protein Coding gene. Diseases associated with FLT3LG include Aplastic Anemia and Brain Cancer. Among its related pathways are Hematopoietic Stem Cell Differentiation Pathways and Lineage-specific Markers and Ras signaling pathway. Gene Ontology (GO) annotations related to this gene include protein homodimerization activity and cytokine activity. Dendritic cells (DCs) provide the key link between innate and adaptive immunity by recognizing pathogens and priming pathogen-specific immune responses. FLT3LG controls the development of DCs and is particularly important for plasmacytoid DCs and CD8-positive classical DCs and their CD103-positive tissue counterparts.