Invitrogen

ATP synthase Monoclonal Antibody (12F4AD8AF8)

Name: ATP synthase Monoclonal Antibody (12F4AD8AF8)
Brand: Invitrogen
SKU: 43-9700

Datasheet

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Questions & Answers

Datasheet

Certificates

Protocols

Questions & Answers

Datasheet

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Protocols

Questions & Answers

Additional Information:

Antibody Testing Data (2)

ATP synthase Antibody in Immunocytochemistry (ICC/IF)

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ATP synthase Antibody (43-9700) in ICC/IF

Immunofluorescence analysis of ATP5A was performed using Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with ATP5A Mouse Monoclonal Antibody (Product # 43-9700) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat a... View More

ATP synthase Antibody in Flow Cytometry (Flow)

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Product Details

43-9700

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

5 µg/mL

Flow Cytometry (Flow)

1 µg/ 10^6 cells

Product Specifications

Species Reactivity

Bovine, Human, Mouse

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

12F4AD8AF8

Immunogen

Bovine Complex V

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

100 µg

Purification

Ammonium sulfate precipitation

Storage buffer

HEPES buffered saline

Contains

0.02% sodium azide

Storage conditions

4°C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533547

Product Specific Information

Positive control: Bovine heart mitochondria

Target Information

Complex V, also called F1F0ATPase or ATP synthase, is responsible for ATP production in oxidative phosphorylation and can work in reverse as a proton pumping ATPase. The enzyme was thought to be localized exclusively to mitochondria. However, it has recently been identified on the plasma membrane of several cell types including hepatocytes where it functions as the HDL receptor, on endothelial cells where it may act as the angiostatin receptor, and on the surface of cancer cells. The enzyme in mammals is composed of 17 subunits, five of which make up the easily detached F1. The remainder subunits are components of two stalk domains and the proton pumping F0 part of the machinery. Two of the subunits of the F0 part are encoded on mitochondrial DNA while the other subunits are nuclear encoded. Mutations in the mitochondrial-encoded subunits of ATP synthase (Complex V) cause OXPHOS disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 6.8 kDa mitochondrial proteolipid protein; 6.8-kDa proteolipid with N-terminal sequence Met-Leu-Gln (MLQ); A6L; alpha subunit ATP synthase isoform precursor (EC 3.6.1.34); ATP synthase alpha chain, mitochondrial; ATP synthase alpha subunit; ATP synthase alpha subunit precursor; ATP synthase B chain, mitochondrial; ATP synthase c subunit; ATP synthase D chain, mitochondrial; ATP synthase e chain, mitochondrial; ATP synthase epsilon chain, mitochondrial; ATP synthase f chain, mitochondrial; ATP synthase F(0) complex subunit 8; ATP synthase F(0) complex subunit a; ATP synthase F(0) complex subunit B1, mitochondrial; ATP synthase F(0) complex subunit C1, mitochondrial; ATP synthase F(0) complex subunit C2, mitochondrial; ATP synthase F(0) complex subunit C3, mitochondrial; ATP synthase F(0) complex subunit e, mitochondrial; ATP synthase F(0) complex subunit f, mitochondrial; ATP synthase F(0) complex subunit g, mitochondrial; ATP synthase F(0) complex subunit j, mitochondrial; ATP synthase F(1) complex catalytic subunit beta, mitochondrial; ATP synthase F(1) complex subunit alpha, mitochondrial; ATP synthase F(1) complex subunit beta, mitochondrial; ATP synthase F(1) complex subunit delta, mitochondrial; ATP synthase F(1) complex subunit epsilon, mitochondrial; ATP synthase F(1) complex subunit gamma, mitochondrial; ATP synthase F1 subunit alpha; ATP synthase F1 subunit beta; ATP synthase F1 subunit delta; ATP synthase F1 subunit epsilon; ATP synthase F1 subunit gamma; ATP synthase g chain, mitochondrial; ATP synthase gamma chain, mitochondrial; ATP synthase gamma-subunit gene encoding a mitochondrial protein; ATP synthase inhibitor protein; ATP synthase lipid-binding protein; ATP synthase lipid-binding protein, mitochondrial; ATP synthase membrane subunit 6.8PL; ATP synthase membrane subunit c locus 1; ATP synthase membrane subunit c locus 2; ATP synthase membrane subunit c locus 3; ATP synthase membrane subunit e; ATP synthase membrane subunit f; ATP synthase membrane subunit g; ATP synthase peripheral stalk subunit b, mitochondrial; ATP synthase peripheral stalk subunit d; ATP synthase peripheral stalk subunit d, mitochondrial; ATP synthase peripheral stalk subunit F6; ATP synthase peripheral stalk subunit F6, mitochondrial; ATP synthase peripheral stalk subunit OSCP, mitochondrial; ATP synthase peripheral stalk-membrane subunit b; ATP synthase proteolipid P1; ATP synthase proteolipid P2; ATP synthase proteolipid P3; ATP synthase proton-transporting mitochondrial F(0) complex subunit B1; ATP synthase proton-transporting mitochondrial F(0) complex subunit C1; ATP synthase proton-transporting mitochondrial F(0) complex subunit C2; ATP synthase proton-transporting mitochondrial F(0) complex subunit C3; ATP synthase subunit 9; ATP synthase subunit ATP5MJ, mitochondrial; ATP synthase subunit ATP5MPL, mitochondrial; ATP synthase subunit b; ATP synthase subunit b, mitochondrial; ATP synthase subunit beta, mitochondrial; ATP synthase subunit d, mitochondrial; ATP synthase subunit delta, mitochondrial; ATP synthase subunit e, mitochondrial; ATP synthase subunit epsilon, mitochondrial; ATP synthase subunit f, mitochondrial; ATP synthase subunit g, mitochondrial; ATP synthase subunit gamma, mitochondrial; ATP synthase subunit h; ATP synthase subunit O; ATP synthase subunit O, mitochondrial; ATP synthase, H+ transporting mitochondrial F1 complex, alpha subunit; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit b; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit c (subunit 9); ATP synthase, H+ transporting, mitochondrial F0 complex, subunit c (subunit 9), isoform 1; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit C1 (subunit 9); ATP synthase, H+ transporting, mitochondrial F0 complex, subunit C2 (subunit 9); ATP synthase, H+ transporting, mitochondrial F0 complex, subunit C3 (subunit 9); ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit E; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit f; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit f, isoform 2; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit G; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle; ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide; ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit; ATP synthase, H+ transporting, mitochondrial F1 complex, epsilon subunit; ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1; ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit; ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit; DNA segment, Chr 12, Wayne State University 28, expressed; ATP synthase, H+ transporting, mitochondrial F1F0, subunit d; ATP synthase, H+ transporting, mitochondrial F1F0, subunit g; ATP synthase, H+ transporting, mitochondrial Fo complex subunit B1; ATP synthase, H+ transporting, mitochondrial Fo complex subunit C1 (subunit 9); ATP synthase, H+ transporting, mitochondrial Fo complex subunit C2 (subunit 9); ATP synthase, H+ transporting, mitochondrial Fo complex subunit C3 (subunit 9); ATP synthase, H+ transporting, mitochondrial Fo complex subunit D; ATP synthase, H+ transporting, mitochondrial Fo complex subunit E; ATP synthase, H+ transporting, mitochondrial Fo complex subunit F2; ATP synthase, H+ transporting, mitochondrial Fo complex subunit F6; ATP synthase, H+ transporting, mitochondrial Fo complex subunit G; ATP synthase, H+ transporting, mitochondrial Fo complex, subunit d; ATP synthase, mitochondrial, C subunit-3; ATP synthase-coupling factor 6, mitochondrial; ATP sythase (F1-ATPase) alpha subunit; Atp5b; ATP5C1; ATP5D; ATP5G1; ATP5G3; ATP5J; ATP5J2; ATP5L; ATP5O; ATPase inhibitor protein; ATPase inhibitor, mitochondrial; ATPase inhibitory factor 1; ATPase protein 9; ATPase subunit 9; ATPase subunit b; ATPase subunit C; ATPase subunit d; ATPase subunit e; ATPase subunit epsilon; ATPase subunit F6; ATPase subunit G; ATPIF1; ATPIP; cell proliferation-inducing protein 47; coupling factor 6; dicyclohexylcarbodiimide (DCCD)-reactive proteolipid subunit; epididymis secretory protein Li 271; epididymis secretory sperm binding protein Li 123m; F(0)F(1)-ATPase; F-1-ATPase beta-subunit precursor; F-ATPase delta subunit; F-ATPase gamma subunit; F-ATPase protein 6; F-ATPase subunit 8; F1 gamma; F1-ATPase beta-subunit; F1F0-ATP synthase g subunit; F1F0-ATP synthase, murine e subunit; F1F0-type ATP synthase subunit g; F1F0-type ATPase subunit f; F1Fo-ATP synthase complex Fo membrane domain f subunit; F1Fo-ATP synthase complex Fo membrane domain g subunit; F1Fo-ATPase synthase f subunit; Fog; H(+)-transporting two-sector ATPase; H+-ATP synthase subunit b; human ATP synthase OSCP subunit; hypothetical protein, clone:2-31; IF(1); IF1; Inhibitor of F(1)F(o)-ATPase; mitochondrial ATP synthase beta subunit; mitochondrial ATP synthase complex delta-subunit precusor; mitochondrial ATP synthase epsilon chain; mitochondrial ATP synthase, coupling factor 6; mitochondrial ATP synthase, delta subunit; mitochondrial ATP synthase, gamma subunit 1; mitochondrial ATP synthase, H+ transporting F1 complex beta subunit; mitochondrial ATP synthase, subunit 9; mitochondrial ATP synthase, subunit C; mitochondrial ATP synthase, subunit F6; mitochondrial ATP synthetase, beta subunit; mitochondrial ATP synthetase, oligomycin-resistant; mitochondrial ATPase; mitochondrial ATPase coupling factor 6; MLQ; My032 protein; Oligomycin sensitivity conferral protein; oligomycin sensitivity conferral protein oscp-like protein; oligomycin sensitivity conferring protein; OSCP; PIG36; PIG47; predicted protein of HQ1574; proliferation-inducing protein 36; Proton-conducting channel, ATP synthase F(0) complex subunit a; Proton-conducting channel, ATP synthase F(0) complex subunit c; unnamed protein product

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Gene Aliases: 0610008F14Rik; 0610009D10Rik; 1110019H14Rik; 1500000I11Rik; 1700094F02Rik; 2410043G19Rik; 2610008D24Rik; 4933437C06Rik; 6.8PL; AA960090; AI876556; APT5H; ATP5; ATP5A; ATP5A1; ATP5A2; ATP5AL2; ATP5B; ATP5C; ATP5C1; ATP5CL1; ATP5D; ATP5E; ATP5F1; ATP5F1A; ATP5F1B; ATP5F1C; ATP5F1D; ATP5F1E; ATP5G; ATP5G1; ATP5G2; ATP5G3; ATP5H; ATP5I; ATP5IF1; ATP5J; ATP5J2; ATP5JG; ATP5JL; ATP5K; ATP5L; ATP5MC1; ATP5MC2; ATP5MC3; ATP5ME; ATP5MF; ATP5MG; ATP5MJ; ATP5MPL; ATP5O; ATP5PB; ATP5PD; ATP5PF; ATP5PO; Atp6; Atp8; Atpase6; ATPE; ATPI; ATPIF1; ATPIP; ATPM; ATPMB; ATPO; ATPQ; ATPSB; AU020773; AV000645; C14orf2; C76477; C85518; CF6; COXPD22; D12Wsu28e; DYTSPG; F6; hATP1; HEL-S-123m; HEL-S-271; HMC08D05; HUMOP2; IP; Lfm-1; Lfm1; MC5DN3; MC5DN4; MC5DN4A; MC5DN4B; MC5DN5; MC5DN7; MLQ; MOM2; MP68; mt-Atp6; mt-Atp8; Mtatp6; Mtatp8; My032; OMR; ORM; OSCP; P3; PIG47; PLPM; PRO1574; PSEC0033

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Function(s)

protein binding nucleotide binding protease binding RNA binding ATP binding ATPase activity adenyl ribonucleotide binding MHC class I protein binding ADP binding angiostatin binding proton-transporting ATP synthase activity, rotational mechanism metal ion binding proton-transporting ATPase activity, rotational mechanism structural molecule activity hydrolase activity enzyme inhibitor activity calmodulin binding enzyme binding ATPase inhibitor activity identical protein binding ATPase binding mitochondrial proton-transporting ATP synthase complex binding lipid binding hydrogen ion transmembrane transporter activity hydrogen ion channel activity macromolecular complex binding estradiol binding calcium ion binding hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances lipoprotein particle receptor activity poly(A) RNA binding protein complex binding hydrogen-exporting ATPase activity, phosphorylative mechanism steroid binding drug binding ATP synthase primary active transporter transporter

Process(es)

negative regulation of endothelial cell proliferation lipid metabolic process ATP biosynthetic process ion transport response to muscle activity ATP synthesis coupled proton transport mitochondrial ATP synthesis coupled proton transport positive regulation of blood vessel endothelial cell migration response to ethanol ATP metabolic process cellular response to dexamethasone stimulus cellular response to nitric oxide hydrogen ion transmembrane transport angiogenesis osteoblast differentiation generation of precursor metabolites and energy negative regulation of cell adhesion involved in substrate-bound cell migration regulation of intracellular pH cellular response to interleukin-7 oxidative phosphorylation aerobic respiration mitochondrial proton-transporting ATP synthase complex assembly response to copper ion heme biosynthetic process erythrocyte differentiation negative regulation of hydrolase activity mitochondrial depolarization reactive oxygen species metabolic process positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway positive regulation of proteolysis involved in cellular protein catabolic process regulation of protein targeting to mitochondrion regulation of ATP metabolic process positive regulation of type 2 mitophagy negative regulation of mitochondrial ATP synthesis coupled proton transport substantia nigra development cellular response to cAMP cellular response to cytokine stimulus transport receptor-mediated endocytosis ATP hydrolysis coupled proton transport proton transport

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ATP synthase Monoclonal Antibody (12F4AD8AF8)

ATP synthase Antibody (43-9700) in ICC/IF

Product Details

43-9700

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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