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Invitrogen

CD85j (ILT2) Monoclonal Antibody (HP-F1), PerCP-eFluor™ 710, eBioscience™

2 Published Figures
3 References
View all (40) CD85j (ILT2) antibodies

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Product Details

46-5129-42

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test
View 3 publications 3 publications
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-eFluor™ 710, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

HP-F1

Conjugate

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

Excitation/Emission Max

482/708 nm View spectra spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10735377

Product Specific Information

Description: The monoclonal antibody HP-F1 recognizes CD85j, also known as ILT2, LILRB1, and LIR1. CD85j is a member of the ILT (immunoglobulin-like transcript)/LIR (leukocyte Ig-like receptor)/MIR (monocyte Ig-like receptor) family. CD85j is a single transmembrane glycoprotein with a long cytoplasmic domain containing 4 ITIMs which signal through interactions with SHP-1. Expression is found on myeloid cells (monocytes and dendritic cells) and some lymphoid cells including, subsets of NK, T and B cells. Expression has been correlated with leukemias such as ALL and CLL. Expression on CD8+ cells correlates with effector cell function and plays an important role in viral infections, including HIV, Ebstein Barr and CMV. The ligands for CD85j are MHC Class I molecules such as HLA-G, A, F, B27, E and F.

The monoclonal antibody HP-F1 has been shown to reduce the amount of CD16- dependent cytolytic activity of functional NK cells.

Applications Reported: This HP-F1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HP-F1 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

PerCP-eFluor 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Target Information

CD85j, also known as a member of the leukocyte immunoglobulin-like receptor (LIR) family, is a single transmembrane glycoprotein with a long cytoplasmic domain containing four immunoreceptor tyrosine-based inhibitory motifs (ITIMs). These motifs signal through interactions with SHP-1, transducing negative signals that inhibit immune response stimulation. CD85j is expressed on myeloid cells such as monocytes and dendritic cells, as well as on subsets of lymphoid cells including NK, T, and B cells. The expression of CD85j is correlated with leukemias such as acute lymphoblastic leukemia (ALL) and chronic lymphocytic leukemia (CLL). On CD8+ cells, CD85j expression is associated with effector cell function and plays a significant role in viral infections, including HIV, Epstein-Barr virus, and cytomegalovirus (CMV). CD85j binds to MHC class I molecules, including HLA-G, A, F, B27, E, and F, on antigen-presenting cells. The receptor helps control inflammatory responses and cytotoxicity, focusing the immune response and limiting autoreactivity. CD85j belongs to the subfamily B class of LIR receptors, which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and multiple transcript variants encoding different isoforms have been identified for this gene. Its gene is located in a cluster at chromosomal region 19q13.4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Bioinformatics

Protein Aliases: CD85 antigen-like family member J; CD85j; Ig-like transcript 2; ILT-2; ILT2a; allelic form of ILT2; ILT2b; alternatively spliced form of ILT2; ILT2c; alternatively spliced form of ILT2; immunoglobulin heavy chain variable region; Immunoglobulin-like transcript 2; leucocyte Ig-like receptor B1; Leukocyte immunoglobulin-like receptor subfamily B member 1; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 1; LIR-1; MIR-7; Monocyte/macrophage immunoglobulin-like receptor 7; myeloid inhibitory receptor 7; unnamed protein product

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Gene Aliases: CD85J; ILT-2; ILT2; LILRB1; LIR-1; LIR1; MIR-7; MIR7; PIR-B; PIRB

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UniProt ID: (Human) Q8NHL6

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Entrez Gene ID: (Human) 10859

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Function(s)
beta-amyloid binding protein binding protein phosphatase 1 binding MHC class Ib protein complex binding MHC class Ib protein binding HLA-A specific inhibitory MHC class I receptor activity HLA-B specific inhibitory MHC class I receptor activity MHC class I receptor activity MHC class Ib receptor activity inhibitory MHC class I receptor activity SH2 domain binding MHC class I protein binding protein homodimerization activity
Process(es)
negative regulation of T cell mediated cytotoxicity positive regulation of defense response to virus by host adaptive immune response T cell proliferation involved in immune response immune system process negative regulation of cytokine production involved in immune response immune response-regulating signaling pathway immune response-inhibiting cell surface receptor signaling pathway Fc receptor mediated inhibitory signaling pathway signal transduction cell surface receptor signaling pathway response to virus positive regulation of gene expression negative regulation of serotonin secretion receptor internalization negative regulation of interferon-beta production negative regulation of interferon-gamma production negative regulation of interleukin-10 production negative regulation of interleukin-12 production negative regulation of tumor necrosis factor production positive regulation of interferon-gamma production negative regulation of mononuclear cell proliferation negative regulation of T cell proliferation positive regulation of apoptotic process negative regulation of cell cycle negative regulation of endocytosis positive regulation of transcription from RNA polymerase II promoter negative regulation of natural killer cell mediated cytotoxicity negative regulation of alpha-beta T cell activation defense response to virus negative regulation of calcium ion transport positive regulation of macrophage cytokine production cellular response to lipopolysaccharide negative regulation of transforming growth factor beta production dendritic cell differentiation interleukin-10-mediated signaling pathway negative regulation of dendritic cell apoptotic process negative regulation of CD8-positive, alpha-beta T cell activation negative regulation of T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell positive regulation of gamma-delta T cell activation involved in immune response negative regulation of dendritic cell differentiation negative regulation of osteoclast development
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