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Invitrogen

IkB zeta Monoclonal Antibody (LK2NAP), PerCP-eFluor™ 710, eBioscience™

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Product Details

46-6801-82

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

0.125 µg/test
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Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PerCP-eFluor™ 710, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

LK2NAP

Conjugate

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

Excitation/Emission Max

482/708 nm View spectra spectra

Form

Liquid

Concentration

0.2 mg/mL

Amount

100 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11149496

Product Specific Information

Description: The LK2NAP monoclonal antibody reacts with mouse IkB zeta (INAP, MAIL). IkBz is a member of the IkB family of cytoplasmic proteins and transcription factors and also shares structural homology with Bcl-3. It is induced in monocytes, macrophages and B cells in response to TLR and IL-1R signaling. In NK cells, IkBz is rapidly upregulated in response to IL-12 and IL-18 and is necessary for production of IFN gamma. In T cells, IkBz is not necessary for IFN gamma and IL-4 production by Th1 and Th2 cells, respectively. However in Th17 cells, IkBz acts in concert with RORa and RORgt to upregulate IL-17A, IL-17F, IL-21, IL-22 and IL-23R expression.

Applications Reported: This LK2NAP antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This LK2NAP antibody has been tested by intracellular staining and flow cytometric analysis of LPS-activated mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.

Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

I-kappa-B-zeta (also known as MAIL and INAP) is an ankyrin-repeat-containing nuclear protein that is highly homologous to the IkappaB family member Bcl-3. Transcription of IkappaBzeta is upregulated by stimulation with TLR ligands, IL-1 and IL-6 in cultured B-lymphocytes and monocytes/macrophages, but only faintly so in T-lymphocytes, fibroblasts, and endothelial cells. IkappaB-zeta preferentially associates with the NF-kappaB subunit p50 rather than p65 and recombinant IkappaB-zeta proteins inhibit the DNA binding of the p65/p50 heterodimer and the p50/p50 homodimer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

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Bioinformatics

Protein Aliases: FLJ30225; FLJ34463; I-kappa-B-zeta; IkappaB-zeta; ikappaBzeta; ikB-zeta; IL-1 inducible nuclear ankyrin-repeat protein; molecule possessing ankyrin-repeats induced by lipopolysaccharide; OTTHUMP00000214340; unnamed protein product

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Gene Aliases: INAP; Mail

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UniProt ID: (Mouse) Q9EST8

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Entrez Gene ID: (Mouse) 80859

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding DNA binding transcription cofactor activity protein binding POU domain binding
Process(es)
toll-like receptor signaling pathway adaptive immune response lymphocyte activation involved in immune response plasma cell differentiation T cell mediated immunity chronic inflammatory response chromatin organization chromatin remodeling transcription from RNA polymerase II promoter inflammatory response protein secretion response to xenobiotic stimulus gene expression regulation of gene expression immunoglobulin mediated immune response cytokine-mediated signaling pathway natural killer cell activation keratinocyte differentiation T cell differentiation positive regulation of defense response response to lipopolysaccharide keratinocyte activation response to cytokine B cell proliferation monocyte activation natural killer cell mediated cytotoxicity mRNA transcription from RNA polymerase II promoter skin development keratinocyte proliferation T-helper 1 cell differentiation isotype switching positive regulation of transcription from RNA polymerase II promoter spleen development homeostasis of number of cells within a tissue positive regulation of inflammatory response regulation of immune response defense response to Gram-negative bacterium T cell receptor signaling pathway B cell receptor signaling pathway response to folic acid defense response to virus establishment of skin barrier response to interleukin-12 response to interleukin-18 transcription preinitiation complex assembly cellular response to lipopolysaccharide cellular response to cytokine stimulus cellular response to transforming growth factor beta stimulus T-helper 17 cell differentiation response to cisplatin inflammatory response to wounding execution phase of apoptosis cellular response to interleukin-17 skin epidermis development response to Gram-positive bacterium epithelial cell apoptotic process B cell receptor apoptotic signaling pathway positive regulation of T-helper 17 cell differentiation
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