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IL-22 Monoclonal Antibody (IL22JOP), PerCP-eFluor™ 710, eBioscience™
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FIGURE: 1 / 20
IL-22 Antibody (46-7222-82) in Flow
Th17 polarized mouse splenocytes either unstimulated (left) or stimulated with Cell Stimulation Cocktail (plus protein transport inhibitors) (500X) (right) were intracellularly stained with Anti-Mouse CD4 FITC (Product # 11-0042-82) and 0.125 µg of Anti-Human/Mouse IL-22 PerCP-eFluor® 710. Cells in the lymphocyte gate were used for analysis.
Product Details
46-7222-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rhesus monkey
Published species
Human,
Mouse,
Rhesus monkey
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
IL22JOP
Conjugate
Excitation/Emission Max
482/708 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The monoclonal antibody IL22JOP reacts with and inhibits the bioactivity of human and mouse IL-22. IL-22 is a 20 kDa member of the IL-10 cytokine family that is secreted primarily by Th17 cells, NK cells, and other T cells. Compared to IL-6 or TGF beta, IL-23 can induce greater levels of IL-22 in in vitro-differentiated Th17 cells. This observation suggests that IL-22 may be secreted by more fully differentiated Th17 cells in vivo. Recently, it was demonstrated that IL-22 could protect hosts from bacterial infection of the lungs and gut. Moreover, it has been reported that anti-CD3/CD28-induced production of IL-22 by PBMCs was elevated significantly in asthma patients compared to control patients. Flow cytometric analysis also showed that the frequencies of IL-17+IL-22+ CD4 T cells were increased in PBMCs from patients with ankylosing spondylitis and rheumatoid arthritis.
IL22JOP is published to recognize rhesus IL-22.
Applications Reported: This IL22JOP antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This IL22JOP antibody has been tested by intracellular staining and flow cytometric analysis of TH17 polarized mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.
Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
IL-22 also known as IL-10-related T-cell derived inducible factor, is an alpha helical cytokine and is considered a member of the IFN-IL-10 family, which includes IL-19, IL-20, IL-24, IL-26, IL-28, IL-29, and the type I and II interferons. IL-22 is produced mainly by activated T cells and NK cells. In humans, the IL-22 gene is located on the q arm of chromosome 12, and is structurally related to IL10. IL-22 acts by engaging the heterodimeric receptor complex consisting of primary receptor IL-22R1 and accessory receptor IL-10R2. IL-22R1 also binds IL-20 and IL-24; IL-10R2 also binds IL-10, IL-27, IL-28, and IL-29. Binding of IL-22 to its receptor complex induces signal transduction, particularly via the JAK-STAT pathway. In addition to the membrane-bound IL-22R1/IL-10R2 complex, a soluble single-chain IL-22 receptor termed IL-22BP has been found to antagonize IL-22 binding and signaling. IL-22 appears not to directly influence immune cells, and major targets of the cytokine appear to be nonimmune cells, such as cells of the skin, digestive and respiratory system, as well as hepatocytes, and keratinocytes. IL-22 has been described as an effector cytokine of the Th17 lineage. Along with IL-17A and IL-17F, IL-22 regulates genes associated with innate immunity of the skin. IL-17A, IL-17F and IL-22 are all co-expressed by Th17 cells, however, they are differentially regulated. The effects of IL-22 include induction of acute phase reactants and antimicrobial proteins, as well as increasing the mobility of keratinocytes. IL-22 is highly expressed during chronic inflammation, and found to activate intracellular kinases and transcription factors. IL-22 is critical for host defense against infections of extracellular pathogens, and promotes wound-healing responses. IL-22 is upregulated in activated T cells. IL-22 has been reported to mediate IL-23-induced acanthosis and dermal inflammation through activation of STAT3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cytokine; Cytokine Zcyto18; il 22; IL-10-related T-cell-derived inducible factor; IL-10-related T-cell-derived-inducible factor; IL-22; IL-22a; IL-TIF; IL-TIF alpha; ILN; ILTIF alpha; Interleukin; interleukin 10-related T cell-derived inducible factor; Interleukin-22; Interleukin-22a; Interleukin22
Gene Aliases: EGK_03903; IL-21; IL-22; IL-22a; IL-D110; IL-TIF; IL22; Il22a; ILTIF; ILTIFa; TIFa; TIFIL-23; UNQ3099/PRO10096; ZCYTO18
UniProt ID: (Human) Q9GZX6, (Mouse) Q9JJY9
Entrez Gene ID: (Human) 50616, (Rhesus monkey) 718047, (Mouse) 50929
acute-phase response
inflammatory response
signal transduction
negative regulation of inflammatory response
response to glucocorticoid
regulation of tyrosine phosphorylation of Stat3 protein
positive regulation of transcription from RNA polymerase II promoter
reactive oxygen species metabolic process
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