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CD357 (AITR/GITR) Monoclonal Antibody (eBioAITR), APC-eFluor™ 780, eBioscience™
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FIGURE: 1 / 1
CD357 (AITR/GITR) Antibody (47-5875-42) in Flow
Normal human peripheral blood cells were unstimulated (left) or stimulated for 72 hours with CD3 and CD28 Monoclonal Antibodies, Functional Grade (Product # 16-0037-85 and 16-0289-85) (right). Cells were then stained with CD25 Monoclonal Antibody, PE (Product # 12-0259-42) and CD357 (AITR/GITR) Monoclonal Antibody, APC-eFluor 780. Total viable cells, as determined by Fixable Viability Dye eFluor® 506 (Produ... View More
Product Details
47-5875-42
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
eBioAITR
Conjugate
Excitation/Emission Max
756/785 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The monoclonal antibody reacts with human AITR, Activation Inducible TNFR family member, an approximately 25 kDa member of the TNFR superfamily. AITR mRNA is detected in lymph node, peripheral blood leukocytes and weakly in spleen. AITR mRNA expression was upregulated within 24 hour on PMA/ionomycin or PHA stimulated PBMC. At the protein level, AITR is expressed by a small population of activated PBMC. AITR associates with TRAF1, TRAF2 and TRAF3 and induces nuclear factor NF-kappaB activation via TRAF2. Recently TL6 (AITRL) has been reported as the ligand for AITR. Interaction of AITR with AITRL is important for cross-talk between T lymphocytes and endothelial cells.
Applications Reported: This eBioAITR antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBioAITR antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (1.0 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633-647 nm). Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.
Target Information
Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), also called GITR or AITR is a protein that in humans is encoded by the TNFRSF18 gene. It is mapped to 1p36.33. This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25 (+)CD4 (+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Activation-inducible TNFR family receptor; AITR; CD357; GITR; TNF receptor homolog; similar to murine glucocorticoid-induced TNFR-related protein; Glucocorticoid-induced TNFR-related protein; PRO364; TNF receptor superfamily activation-inducible protein; Tumor necrosis factor receptor superfamily member 18; UNQ319; variant (4) contains an extra coding segment, which leads to a frame shift, compared to variant 1 and 3; resulting protein (isoform 4) contains a transmembrane domain (identical to variant 1 and 3) and a longer cytoplasmic domain with a sequence different
Gene Aliases: AITR; CD357; ENERGEN; GITR; GITR-D; TNFRSF18; UNQ319/PRO364
UniProt ID: (Human) Q9Y5U5
Entrez Gene ID: (Human) 8784
Disclaimer
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