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CD337 (NKp30) Monoclonal Antibody (AF29-4D12), eFluor™ 450, eBioscience™
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FIGURE: 1 / 2
CD337 (NKp30) Antibody (48-3379-42) in Flow
Staining of normal human peripheral blood cells with Anti-Human CD56 (NCAM) APC (Product # 17-0567-42) and Mouse IgG1 K Isotype Control eFluor® 450 (Product # 48-4714-82) (left) or Anti-Human CD337 (NKp30) eFluor® 450 (right). Cells in the lymphocyte gate were used for analysis.
Product Details
48-3379-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Non-human primate,
Rhesus monkey
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
AF29-4D12
Conjugate
Excitation/Emission Max
405/445 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This AF29-4D12 monoclonal antibody reacts with human CD337, which is also known as NKp30. Expressed on NK cells, CD337 is the predominant member of the natural cytotoxicity receptor family that also includes NKp46 and NKp44. CD337 is involved in activating and expanding resting NK cells following interaction with dendritic cells to eliminate tumor, virus-infected, and immature dendritic cells. Several ligands for this receptor have been reported, including B7-H6.
The AF29-4D12 antibody has been reported to crossreact to rhesus macaque.
Applications Reported: This AF29-4D12 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This AF29-4D12 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The protein encoded by this gene is a natural cytotoxicity receptor (NCR) that may aid NK cells in the lysis of tumor cells. The encoded protein interacts with CD3-zeta (CD247), a T-cell receptor. A single nucleotide polymorphism in the 5' untranslated region of this gene has been associated with mild malaria suceptibility. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010].
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: Activating natural killer receptor p30; activating NK-A1 receptor; CD337; lymphocyte antigen 117; Natural cytotoxicity triggering receptor 3; Natural killer cell p30-related protein; natural killer cell receptor; NK-p30
Gene Aliases: 1C7; CD337; LY117; MALS; NCR3; NKp30
UniProt ID: (Human) O14931, (Rhesus monkey) Q8MJ02
Entrez Gene ID: (Human) 259197, (Rhesus monkey) 715574
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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