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EOMES Monoclonal Antibody (Dan11mag), eFluor™ 450, eBioscience™
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FIGURE: 1 / 53
EOMES Antibody (48-4875-82) in Flow
C57Bl/6 splenocytes were surface stained with Anti-Mouse NK1-1 APC (Product # 17-5941-82) followed by fixation and permeabilization using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Cells were intracellularly stained with 0.25 µg of Rat IgG2a K Isotype Control eFluor® 450 (Product # 48-4321-82) (left) or 0.25 µg of Anti-Mouse EOMES eFluor® 450 (right). Cells in the lymphocyte gate were used for analysis.
Product Details
48-4875-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Human,
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
Dan11mag
Conjugate
Excitation/Emission Max
405/445 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This Dan11mag antibody recognizes Eomesodermin (EOMES), also known as T-box brain 2 (TBR2). Eomes is a T-box transcription factor that is highly homologous to T-bet, which is essential during trophoblast development and gastrulation in most vertebrates. In the immune system, Eomes controls the differentiation of effector and memory CD8+ T cells, as well as natural killer (NK) cells. Expression of Eomes in these cells correlates with high expression of CD122, the common beta-chain of the IL-2R and IL-15R.
Applications Reported: This Dan11mag antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This Dan11mag antibody has been tested by intracellular staining and flow cytometric analysis of mouse splenocytes using the Foxp3/Transcription Factor Buffer Set (Product # 00-5523-00) and protocol. Please see BestProtocols® Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochrome.
Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
TBR2 is a member of a conserved protein family that shares a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. A similiar protein disrupted in mice is shown to be essential during trophoblast development and gastrulation. TBR2 plays a role in brain development and is required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Eomesodermin homolog; T-box brain protein 2; T-brain-2
Gene Aliases: C77258; Eomes; TBR-2; Tbr2
UniProt ID: (Mouse) O54839
Entrez Gene ID: (Mouse) 13813
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II activating transcription factor binding
transcriptional repressor activity, RNA polymerase II transcription factor binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
sequence-specific DNA binding
Rel homology transcription factor
negative regulation of transcription from RNA polymerase II promoter
endoderm formation
mesoderm formation
endodermal cell fate specification
blastocyst development
trophectodermal cell differentiation
CD8-positive, alpha-beta T cell differentiation involved in immune response
immune system process
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
multicellular organism development
gastrulation
brain development
cardioblast differentiation
regulation of gene expression
stem cell population maintenance
olfactory bulb development
cerebral cortex regionalization
cerebral cortex neuron differentiation
cell differentiation
interferon-gamma production
skeletal muscle cell differentiation
positive regulation of cell differentiation
regulation of neuron differentiation
positive regulation of transcription from RNA polymerase II promoter
regulation of neurogenesis
cell differentiation involved in embryonic placenta development
mesodermal to mesenchymal transition involved in gastrulation
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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