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Progesterone Receptor Monoclonal Antibody (KMC912), eFluor™ 660, eBioscience™
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FIGURE: 1 / 4
Progesterone Receptor Antibody (50-9764-80) in ICC/IF
Immunofluorescence analysis of Progesterone receptor was performed using 70 % confluent log phase T-47D cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1 % Triton™ X-100 for 15 minutes, and blocked with 2 % BSA for 1 hour at room temperature. The cells were labeled with Progesterone Receptor Monoclonal Antibody (KMC912), eFluor™ 660, eBioscience™ (Product # 50-9764-... View More
Product Details
50-9764-80
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
KMC912
Conjugate
Excitation/Emission Max
651/668 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
25 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This KMC912 monoclonal antibody reacts with human progesterone receptor (PgR, PR), a member of a superfamily of nuclear receptors that are ligand-dependent transcriptional regulators. The human PgR exists in alpha and beta forms, 94 kDa and 120 kDa respectively. In most human cells, the alpha and beta forms are expressed at similar levels and predominately form heterodimers. Progestin binding to PgR causes a conformational change, allowing dissociation of bound chaperone proteins and subsequent dimerization with either PgRa or PgRb. Following activation, dimerized PgR can directly bind to DNA through progestin response elements (PRE) leading to chromatin remodeling and subsequent downregulation or transcription of the target gene. The PgR plays a key role in controlling gene expression in breast, uterine, brain, and cardiovascular tissue during development. The presence of the PgR in breast tissue is indicative of improved survival and a better response to endocrine therapy. In breast and endometrial cancer progression, a predominance of either the alpha or beta form occurs, suggesting disregulation in the PgRa:PgRb ratio is an early event in cancer. In cases of ductal carcinoma in situ and invasive ductal carcinoma, there is predominance of the alpha form while in uterine cancer a loss of either form is common.
Applications Reported: This KMC912 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry.
Applications Tested: This KMC912 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 20 µg/mL. This KMC912 antibody has also been tested by immunocytochemistry of methanol-fixed and permeabilized human cells and can be used at less than or equal to 20 µg/mL. This KMC912 antibody has also been tested by intracellular staining followed by flow cytometric analysis of BT474 cells using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to BestProtocols®: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online. This can be used at less than or equal to 1 µg/test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochrome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The progesterone receptor (PR) is a member of the steroid family of nuclear receptors. The PR mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. PR is found as a 94 kDa protein (Form A) or a 120 kDa protein (Form B) due to the use of alternative translation initiation sites. PR-B is the transcriptionally active form and is responsible for activating genes for the maintenance of the endometrium, maintenance of pregnancy, and inhibition of ovulation. PR-A is identical to PR-B except for a 165 amino acid deletion at the N-terminus. This deletion exposes a 140 amino acid inhibitory domain (ID) that acts as a repressor of steroid hormone transcriptional activity. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription. A Null mutation in the PGR gene leads to pleiotrophic reproductive abnormalities.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Nuclear receptor subfamily 3 group C member 3; PR; Progesterone receptor; unnamed protein product
Gene Aliases: NR3C3; PGR; PR
UniProt ID: (Human) P06401, (Rat) Q63449
Entrez Gene ID: (Human) 5241, (Rat) 25154
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcription coactivator binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
steroid hormone receptor activity
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
receptor binding
steroid binding
protein binding
zinc ion binding
lipid binding
enzyme binding
estrogen response element binding
identical protein binding
sequence-specific DNA binding
metal ion binding
ATPase binding
sequence-specific double-stranded DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
hormone binding
ovulation from ovarian follicle
luteinization
epithelial cell maturation
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
positive regulation of cell proliferation
negative regulation of gene expression
positive regulation of smooth muscle cell migration
diterpenoid metabolic process
intracellular receptor signaling pathway
mammary gland development
response to progesterone
response to cocaine
negative regulation of apoptotic process
steroid hormone mediated signaling pathway
response to estrogen
estrous cycle
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
regulation of epithelial cell proliferation
progesterone receptor signaling pathway
female mating behavior
tertiary branching involved in mammary gland duct morphogenesis
cellular response to gonadotropin stimulus
cellular response to follicle-stimulating hormone stimulus
cellular response to luteinizing hormone stimulus
glandular epithelial cell maturation
signal transduction
cell-cell signaling
positive regulation of gene expression
intracellular steroid hormone receptor signaling pathway
paracrine signaling
lung alveolus development
maintenance of protein location in nucleus
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