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Invitrogen
Cardiotrophin 1 Polyclonal Antibody, PeproTech®
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FIGURE: 1 / 3
Cardiotrophin 1 Antibody (500-P101-50UG) in WB
Western Blot: To detect Human Cardiotrophin-1 by Western Blot analysis Cardiotrophin 1 Polyclonal Antibody (Product # 500-P101-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Cardiotrophin-1 is 1.5-3.0 ng/lane, under reducing or non-reducing conditions.
Product Details
500-P101-50UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human Cardiotrophin-1
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: SRREGSLEDP QTDSSVSLLP HLEAKIRQTH SLAHLLTKYA EQLLQEYVQL QGDPFGLPSF SPPRLPVAGL SAPAPSHAGL PVHERLRLDA AALAALPPLL DAVCRRQAEL NPRAPRLLRR LEDAARQARA LGAAVEALLA ALGAANRGPR AEPPAATASA ASATGVFPAK VLGLRVCGLY REWLSRTEGD LGQLLPGGSA.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Cardiotrophin-1. Anti-Human Cardiotrophin-1-specific antibody was purified by affinity chromatography employing an immobilized Human Cardiotrophin-1 matrix.
Sandwich ELISA: To detect Human Cardiotrophin-1 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human Cardiotrophin-1 (500-P101Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Cardiotrophin-1.
Western Blot: To detect Human Cardiotrophin-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Cardiotrophin-1 is 1.5-3.0 ng/lane, under reducing or non-reducing conditions.
Target Information
Cardiotrophin-1 (CT-1) is a member of the IL-6 family of cytokines, which signal through gp130 receptor complexes. gp130 complexes with several different receptor subunits to transmit signals from Cardiotrophin-1, IL-6, LIF, OSM, CNTF and IL-11. Cardiotrophin-1 binds to and activates the leukemia inhibitory factor (LIF) receptor/gp130 receptor complex and has been shown to induce hypertrophy in cardiac myocytes in vitro. Cardiotrophin-1, a secreted protein expressed at high levels in myocardium during cardiogenesis, has been shown to promote proliferation and survival of embryonic cardiomyocytes, suggesting a role for Cardiotrophin-1 in the activation of gp130 during cardiac development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cardiophin 1; Cardiotrophin; Cardiotrophin-1; Cardiotrophin1; CT 1; CT-1; CT-1CT1; CTF 1
Gene Aliases: CT-1; CT1; CTF1
UniProt ID: (Human) Q16619
Entrez Gene ID: (Human) 1489
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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