Search
Invitrogen
MCP-2 Polyclonal Antibody, PeproTech®
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
{{$ctrl.translations['antibody.pdp.skuNotes.additionalInformation']}}:
FIGURE: 1 / 3
MCP-2 Antibody (500-P127-50UG) in WB
Western Blot: To detect Murine MCP-2 (CCL8) by Western Blot analysis MCP-2 Polyclonal Antibody (Product # 500-P127-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine MCP-2 (CCL8) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
500-P127-50UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Murine MCP-2 (CCL8)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: GPDKAPVTCC FHVLKLKIPL RVLKSYERIN NIQCPMEAVV FQTKQGMSLC VDPTQKWVSE YMEILDQKSQ ILQP.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine MCP-2 (CCL8). Anti-Murine MCP-2 (CCL8)-specific antibody was purified by affinity chromatography employing an immobilized Murine MCP-2 (CCL8) matrix.
Sandwich ELISA: To detect Murine MCP-2 (CCL8) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine MCP-2 (CCL8) (500-P127Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine MCP-2 (CCL8).
Western Blot: To detect Murine MCP-2 (CCL8) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine MCP-2 (CCL8) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
This antimicrobial gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes, lymphocytes, basophils and eosinophils. By recruiting leukocytes to sites of inflammation this cytokine may contribute to tumor-associated leukocyte infiltration and to the antiviral state against HIV infection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: chemokine (C-C motif) ligand 8; monocyte chemoattractant protein-2; monocyte chemotactic protein 2; small-inducible cytokine A8; unnamed protein product
Gene Aliases: 1810063B20Rik; HC14; MCP-2; Mcp2; Scya8
UniProt ID: (Mouse) Q9Z121
Entrez Gene ID: (Mouse) 20307
calcium ion transport
cellular calcium ion homeostasis
exocytosis
inflammatory response
immune response
cell-cell signaling
positive regulation of cell migration
killing of cells of other organism
negative regulation by host of viral genome replication
positive regulation of myoblast differentiation
eosinophil chemotaxis
antimicrobial humoral immune response mediated by antimicrobial peptide
chemokine-mediated signaling pathway
positive regulation of myoblast fusion
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support