Search
Invitrogen
TNFR1 (soluble) Polyclonal Antibody, PeproTech®
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 3
TNFR1 (soluble) Antibody (500-P143-100UG) in WB
Western Blot: To detect Human sTNF Receptor Type I by Western Blot analysis TNFR1 (soluble) Polyclonal Antibody (Product # 500-P143-1MG) can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human sTNF Receptor Type I is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P143-100UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human sTNF Receptor Type I
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: MDSVCPQGKY IHPQNNSICC TKCHKGTYLY NDCPGPGQDT DCRECESGSF TASENHLRHC LSCSKCRKEM GQVEISSCTV DRDTVCGCRK NQYRHYWSEN LFQCFNCSLC LNGTVHLSCQ EKQNTVCTCH AGFFLRENEC VSCSNCKKSL ECTKLCLPQI EN.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human sTNF Receptor Type I. Anti-Human sTNF Receptor Type I-specific antibody was purified by affinity chromatography employing an immobilized Human sTNF Receptor Type I matrix.
Sandwich ELISA: To detect Human sTNF Receptor Type I by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human sTNF Receptor Type I (500-P143Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human sTNF Receptor Type I.
Western Blot: To detect Human sTNF Receptor Type I by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human sTNF Receptor Type I is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
TNFR1 (Tumor necrosis factor receptor 1) belongs to the tumor necrosis factor superfamily, and is one of the major TNF-alpha receptors. TNFR1 plays an important role in mediating, in cytokine mediated signaling, positive regulation of the NF-Kb pathway, positive regulation of angiogenesis, and negative regulation of gene expression. The extracellular domain of TNFR1 is also released into the circulatory system as soluble TNFR1 (sTNFR1). In humans, the TNFR1 gene is located on chromosome 12. Anti-apoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with TNFR1, and thus play regulatory roles in the signal transduction mediated by the receptor. Germline mutations of the extracellular domains of TNFR1 were found to be associated with the autosomal dominant periodic fever syndrome, and the impaired receptor clearance is thought to be a mechanism of the disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: CD120a; CD120a antigen; MGC19588; n-smase activation assoc. factor; p55; p60; p62; p63; sCD120a; soluble CD120a; soluble TNFR1; sTNF RI; sTNFR I; sTNFR1; sTNFRI; TNF alpha gene; TNF-R1; TNF-RI; TNFR; TNFR I; TNFR-I; TNFR1 beta; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor; Tumor necrosis factor receptor 1; Tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor; unnamed protein product
Gene Aliases: CD120a; FPF; p55; p55-R; p60; TBP1; TNF-R; TNF-R-I; TNF-R55; TNFAR; TNFR1; TNFR55; TNFR60; TNFRSF1A
UniProt ID: (Human) P19438
Entrez Gene ID: (Human) 7132
aortic valve development
pulmonary valve development
negative regulation of extracellular matrix constituent secretion
transcription from RNA polymerase II promoter
prostaglandin metabolic process
apoptotic process
defense response
inflammatory response
signal transduction
cell surface receptor signaling pathway
JAK-STAT cascade
extrinsic apoptotic signaling pathway via death domain receptors
intrinsic apoptotic signaling pathway in response to DNA damage
regulation of gene expression
positive regulation of macromolecule biosynthetic process
negative regulation of cardiac muscle hypertrophy
regulation of tumor necrosis factor-mediated signaling pathway
cytokine-mediated signaling pathway
tumor necrosis factor-mediated signaling pathway
positive regulation of cellular amide metabolic process
NIK/NF-kappaB signaling
defense response to bacterium
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of lipid metabolic process
positive regulation of transcription from RNA polymerase II promoter
negative regulation of inflammatory response
positive regulation of inflammatory response
regulation of developmental process
regulation of multicellular organismal process
cellular response to mechanical stimulus
protein localization to plasma membrane
apoptotic signaling pathway
positive regulation of execution phase of apoptosis
positive regulation of apoptotic process involved in morphogenesis
regulation of establishment of endothelial barrier
regulation of membrane lipid metabolic process
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support