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Invitrogen
IL-2 Polyclonal Antibody, Biotin, PeproTech®
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FIGURE: 1 / 3
IL-2 Antibody (500-P22BT-25UG) in WB
Western Blot: To detect hIL-2 by Western Blot analysis IL-2 Polyclonal Antibody, Biotin (Product # 500-P22BT-500UG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hIL-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P22BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human IL-2
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Amount
25 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: MAPTSSSTKK TQLQLEHLLL DLQMILNGIN NYKNPKLTRM LTFKFYMPKK ATELKHLQCL EEELKPLEEV LNLAQSKNFH LRPRDLISNI NVIVLELKGS ETTFMCEYAD ETATIVEFLN RWITFAQSII STLT.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-2. Anti-Human IL-2-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hIL-2 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IL-2 (500-P22) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hIL-2.
Western Blot: To detect hIL-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hIL-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P22BT-1MG will be provided as 2 x 500 µg
Target Information
Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: aldesleukin; cytokine; H-IL-2; il 2; IL-2; ILN; Interleukin; Interleukin-2; Interleukin2; Involved in regulation of T cell clonal expansion; involved in regulation of T-cell clonal expansion; lymphokine; M-IL-2; T cell growth factor; T-cell growth factor; T-cell growth factor (TCGF); TCGF
Gene Aliases: IL-2; IL2; lymphokine; TCGF
UniProt ID: (Human) P60568
Entrez Gene ID: (Human) 3558
adaptive immune response
leukocyte activation involved in immune response
immune system process
positive regulation of immunoglobulin production
negative regulation of B cell apoptotic process
transcription from RNA polymerase II promoter
immune response
cell adhesion
G-protein coupled receptor signaling pathway
phospholipase C-activating G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
cell-cell signaling
positive regulation of cell proliferation
gene expression
natural killer cell activation
T cell differentiation
positive regulation of cell growth
positive regulation of B cell proliferation
positive regulation of interferon-gamma production
positive regulation of interleukin-17 production
positive regulation of tissue remodeling
interleukin-2-mediated signaling pathway
T cell proliferation
positive regulation of T cell proliferation
positive regulation of activated T cell proliferation
negative regulation of apoptotic process
response to ethanol
positive regulation of T cell differentiation
positive regulation of regulatory T cell differentiation
positive regulation of lymphocyte differentiation
positive regulation of transcription from RNA polymerase II promoter
regulation of T cell homeostatic proliferation
lymphocyte proliferation
positive regulation of isotype switching to IgG isotypes
negative regulation of lymphocyte proliferation
negative regulation of inflammatory response
positive regulation of inflammatory response
activated T cell proliferation
positive regulation of dendritic spine development
extrinsic apoptotic signaling pathway in absence of ligand
receptor signaling pathway via STAT
positive regulation of plasma cell differentiation
response to tacrolimus
negative regulation of T-helper 17 cell differentiation
regulation of CD4-positive, alpha-beta T cell proliferation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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