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Invitrogen
IGFBP5 Polyclonal Antibody, Biotin, PeproTech®
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FIGURE: 1 / 3
IGFBP5 Antibody (500-P232BT-50UG) in WB
Western Blot: To detect hIGF-BP5 by Western Blot analysis IGFBP5 Polyclonal Antibody, Biotin (Product # 500-P232BT-500UG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hIGF-BP5 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P232BT-50UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human IGF-BP5
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: MLGSFVHCEP CDEKALSMCP PSPLGCELVK EPGCGCCMTC ALAEGQSCGV YTERCAQGLR CLPRQDEEKP LHALLHGRGV CLNEKSYREQ VKIERDSREH EEPTTSEMAE ETYSPKIFRP KHTRISELKA EAVKKDRRKK LTQSKFVGGA ENTAHPRIIS APEMRQESEQ GPCRRHMEAS LQELKASPRM VPRAVYLPNC DRKGFYKRKQ CKPSRGRKRG ICWCVDKYGM KLPGMEYVDG DFQCHTFDSS NVS.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IGF-BP5. Anti-Human IGF-BP5-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hIGF-BP5 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IGF-BP5 (500-P232) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hIGF-BP5.
Western Blot: To detect hIGF-BP5 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hIGF-BP5 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P232BT-1MG will be provided as 2 x 500 µg
Target Information
The insulin-like growth factor-binding proteins, or IGFBPs, are a family of homologous proteins that have co-evolved with the IGFs. They serve not only as shuttle molecules for the soluble IGFs, but also confer a level of regulation to the IGF signaling system. Physical association of the IGFBPs with IGF influences the bio-availability of the growth factors, as well as their concentration and distribution in the extracellular environment. In addition, the IGFBPs appear to have biological activity independent of the IGFs. Seven IGFBPs have thus far been described, each differing in their tissue distribution, half-lives and modulation of IGF interactions with their receptors. For instance, IGFBP1 is negatively regulated by insulin production. The IGFBP1 gene is expressed at a high level during fetal liver development and in response to nutritional changes and diabetes. It has been suggested that IGFBP2 functions as chaperone, escorting IGFs to their target tissues. It is expressed in several human tissues including fetal eye and fetal brain. IGFBP3 is the most abundant IGFBP and is complexed with roughly 80% of the serum IGFs. Both IGFBP3 and IGFBP4 are released by dermal fibroblasts in response to incision injury. IGFBP5 is secreted by myoblasts and may play a key role in muscle differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: IBP 5; IBP-5; IGF BP5; IGF-binding protein 5; IGFBP; IGFBP 5; IGFBP-5; IGFBP5; Insulin like growth factor binding protein; Insulin-like growth factor-binding protein 5; unnamed protein product
Gene Aliases: IBP5; IGFBP5
UniProt ID: (Human) P24593
Entrez Gene ID: (Human) 3488
regulation of cell growth
osteoblast differentiation
signal transduction
female pregnancy
cell proliferation
negative regulation of smooth muscle cell migration
negative regulation of translation
negative regulation of cell migration
hair follicle morphogenesis
intracellular signal transduction
regulation of growth
glucose homeostasis
regulation of insulin-like growth factor receptor signaling pathway
positive regulation of insulin-like growth factor receptor signaling pathway
negative regulation of insulin-like growth factor receptor signaling pathway
type B pancreatic cell proliferation
negative regulation of osteoblast differentiation
negative regulation of growth
insulin-like growth factor receptor signaling pathway
lung alveolus development
negative regulation of smooth muscle cell proliferation
striated muscle cell differentiation
positive regulation of protein kinase B signaling
mammary gland involution
response to growth hormone
cellular response to cAMP
negative regulation of muscle tissue development
negative regulation of skeletal muscle hypertrophy
positive regulation of vascular smooth muscle cell proliferation
positive regulation of vascular associated smooth muscle cell migration
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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