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Invitrogen
IL-4 Polyclonal Antibody, PeproTech®
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FIGURE: 1 / 3
IL-4 Antibody (500-P24-100UG) in WB
Western Blot: To detect Human IL-4 by Western Blot analysis IL-4 Polyclonal Antibody (Product # 500-P24-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P24-100UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human IL-4
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-4. Anti-Human IL-4-specific antibody was purified by affinity chromatography employing an immobilized Human IL-4 matrix.
Sandwich ELISA: To detect Human IL-4 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IL-4 (500-P24Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-4.
Western Blot: To detect Human IL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: B cell growth factor 1; B-cell stimulatory factor 1; B_cell stimulatory factor 1; Binetrakin; BSF-1; H-IL-4; IL-4; IL-4v2; IL4; ILN; Interleukin; interleukin 4; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402; Pitrakinra
Gene Aliases: BCGF-1; BCGF1; BSF-1; BSF1; IL-4; IL4
UniProt ID: (Human) P05112
Entrez Gene ID: (Human) 3565
negative regulation of transcription from RNA polymerase II promoter
immune response
JAK-STAT cascade
cholesterol metabolic process
positive regulation of cell proliferation
positive regulation of gene expression
negative regulation of epithelial cell migration
positive regulation of macroautophagy
B cell differentiation
positive regulation of cell migration
positive regulation of B cell proliferation
negative regulation of tumor necrosis factor production
positive regulation of interleukin-10 production
positive regulation of interleukin-13 production
interleukin-4-mediated signaling pathway
type 2 immune response
positive regulation of T cell proliferation
T cell activation
B cell activation
macrophage activation
myeloid dendritic cell differentiation
negative regulation of apoptotic process
regulation of isotype switching
leukocyte activation
positive regulation of MHC class II biosynthetic process
positive regulation of T cell differentiation
negative regulation of osteoclast differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of receptor-mediated endocytosis
positive regulation of isotype switching to IgE isotypes
positive regulation of isotype switching to IgG isotypes
negative regulation of inflammatory response
regulation of immune response
regulation of multicellular organismal process
regulation of primary metabolic process
dendritic cell differentiation
positive regulation of cold-induced thermogenesis
neuroinflammatory response
negative regulation of neuroinflammatory response
positive regulation of beta-amyloid clearance
positive regulation of cellular respiration
negative regulation of complement-dependent cytotoxicity
negative regulation of cellular response to transforming growth factor beta stimulus
negative regulation of endothelial cell apoptotic process
positive regulation of T-helper 2 cell cytokine production
positive regulation of ATP biosynthetic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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