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Invitrogen
TL1A Polyclonal Antibody, Biotin, PeproTech®
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FIGURE: 1 / 1
TL1A Antibody (500-P240BT-25UG) in ELISA
Sandwich ELISA: To detect hTL-1A by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of TL1A Polyclonal Antibody, Biotin (Product # 500-P240BT-500UG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech TL1A Polyclonal Antibody (Product # 500-P240-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hTL-1A.
Product Details
500-P240BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived, 22.0 kDa Recombinant Human TL-1A
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Amount
25 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: QLRAQGEASV QFQALKGQEF APSHQQVYAP LRADGDKPRA HLTVVRQTPT QHFKNQFPAL HWEHELGLAF TKNRMNYTNK FLLIPESGDY FIYSQVTFRG MTSECSEIRQ AGRPNKPDSI TVVITKVTDS YPEPTQLLMG TKSVCEVGSN WFQPIYLGAM FSLQEGDKLM VNVSDISLVD YTKEDKTFFG AFLL.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human TL-1A. Anti-Human TL-1A-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hTL-1A by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human TL-1A (500-P240) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hTL-1A.
Western Blot: To detect hTL-1A by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hTL-1A is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P240BT-1MG will be provided as 2 x 500 µg
Target Information
The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is abundantly expressed in endothelial cells, but is not expressed in either B or T cells. The expression of this protein is inducible by TNF and IL-1 alpha. This cytokine is a ligand for receptor TNFRSF25 and decoy receptor TNFRSF21/DR6. It can activate NF-kappaB and MAP kinases, and acts as an autocrine factor to induce apoptosis in endothelial cells. This cytokine is also found to inhibit endothelial cell proliferation, and thus may function as an angiogenesis inhibitor. An additional isoform encoded by an alternatively spliced transcript variant has been reported but the sequence of this transcript has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cytokine; MGC129934; MGC129935; TNF ligand-related molecule 1; TNF superfamily ligand TL1A; tumor necrosis factor (ligand) superfamily, member 15; tumor necrosis factor ligand 1B; Tumor necrosis factor ligand superfamily member 15; tumor necrosis factor superfamily member 15; unnamed protein product; Vascular endothelial cell growth inhibitor; vascular endothelial growth inhibitor; vascular endothelial growth inhibitor-192A
Gene Aliases: TL1; TL1A; TNFSF15; TNLG1B; VEGI; VEGI192A
UniProt ID: (Human) O95150
Entrez Gene ID: (Human) 9966
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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