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Invitrogen
CTGF Polyclonal Antibody, PeproTech®
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FIGURE: 1 / 4
CTGF Antibody (500-P252-50UG) in WB
Western Blot: To detect Human CTGF by Western Blot analysis CTGF Polyclonal Antibody (Product # 500-P252-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human CTGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P252-50UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse,
Rat
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human CTGF
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: GKKCIRTPKI SKPIKFELSG CTSMKTYRAK FCGVCTDGRC CTPHRTTTLP VEFKCPDGEV MKKNMMFIKT CACHYNCPGD NDIFESLYYR KMYGDMA.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human CTGF. Anti-Human CTGF-specific antibody was purified by affinity chromatography employing an immobilized Human CTGF matrix.
Sandwich ELISA: To detect Human CTGF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human CTGF (500-P252Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human CTGF.
Western Blot: To detect Human CTGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human CTGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
Connective Tissue Growth Factor (CTGF), also called CCN2, is a member of the CCN family. CTGF is a secreted protein produced by umbilical veins and vascular endothelial cells. CTGF possesses an Insulin-like Growth Factor (IGF)-binding domain, a thrombospondin type 1 domain, and a cysteine knot region. CTGF plays important roles in the proliferation and differentiation of chondrocytes, induces angiogenesis, and promotes cell adhesion in fibroblasts, endothelial, and epithelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 2-Nov; CCN family member 2; Cellular communication network factor 2; Connective tissue growth factor; CTGF; H-CTGF; Hypertrophic chondrocyte-specific protein 24; IBP-8; IGF-binding protein 8; Insulin-like growth factor-binding protein 8; MGC102839; unnamed protein product
Gene Aliases: CCN2; CTGF; HCS24; IBP-8; IGFBP8; KMD; NOV2; SEMDLSL
UniProt ID: (Human) P29279
Entrez Gene ID: (Human) 1490
cartilage condensation
ossification
angiogenesis
osteoblast differentiation
tissue homeostasis
chondrocyte differentiation
cell adhesion
cell-matrix adhesion
signal transduction
integrin-mediated signaling pathway
fibroblast growth factor receptor signaling pathway
epidermis development
response to wounding
negative regulation of gene expression
cell migration
lung development
regulation of chondrocyte differentiation
chondrocyte proliferation
positive regulation of cell differentiation
positive regulation of JNK cascade
regulation of developmental process
positive regulation of stress fiber assembly
connective tissue development
positive regulation of ERK1 and ERK2 cascade
DNA biosynthetic process
reactive oxygen species metabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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