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Invitrogen
Maspin Polyclonal Antibody, Biotin, PeproTech®
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FIGURE: 1 / 3
Maspin Antibody (500-P270BT-25UG) in WB
Western Blot: To detect hMaspin by Western Blot analysis Maspin Polyclonal Antibody, Biotin (Product # 500-P270BT-500UG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hMaspin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P270BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human Maspin
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Amount
25 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: MDALQLANSA FAVDLFKQLC EKEPLGNVLF SPICLSTSLS LAQVGAKGDT ANEIGQVLHF ENVKDIPFGF QTVTSDVNKL SSFYSLKLIK RLYVDKSLNL STEFISSTKR PYAKELETVD FKDKLEETKG QINNSIKDLT DGHFENILAD NSVNDQTKIL VVNAAYFVGK WMKKFPESET KECPFRLNKT DTKPVQMMNM EATFCMGNID SINCKIIELP FQNKHLSMFI LLPKDVEDES TGLEKIEKQL NSESLSQWTN PSTMANAKVK LSIPKFKVEK MIDPKACLEN LGLKHIFSED TSDFSGMSET KGVALSNVIH KVCLEITEDG GDSIEVPGAR ILQHKDELNA DHPFIYIIRH NKTRNIIFFG KFCSP.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Maspin. Anti-Human Maspin-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hMaspin by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Maspin (500-P270) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hMaspin.
Western Blot: To detect hMaspin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hMaspin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P270BT-1MG will be provided as 2 x 500 µg
Target Information
SERPINB5 is a tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Maspin; Peptidase inhibitor 5; PI-5; protease inhibitor 5 (maspin); serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5; Serpin B5; serpin peptidase inhibitor, clade B (ovalbumin), member 5; SERPINB5-N; unnamed protein product
Gene Aliases: maspin; PI5; SERPINB5
UniProt ID: (Human) P36952
Entrez Gene ID: (Human) 5268
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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