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Invitrogen
IFN beta Polyclonal Antibody, Biotin, PeproTech®
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FIGURE: 1 / 3
IFN beta Antibody (500-P32BBT-50UG) in WB
Western Blot: To detect Human IFN-beta by Western Blot analysis IFN beta Polyclonal Antibody, Biotin (Product # 500-P32BBT-500UG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IFN-beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P32BBT-50UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human IFN-beta
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: MSYNLLGFLQ RSSNFQCQKL LWQLNGRLEY CLKDRMNFDI PEEIKQLQQF QKEDAALTIY EMLQNIFAIF RQDSSSTGWN ETIVENLLAN VYHQINHLKT VLEEKLEKED FTRGKLMSSL HLKRYYGRIL HYLKAKEYSH CAWTIVRVEI LRNFYFINRL TGYLRN.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IFN-beta. Anti-Human IFN-beta-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human IFN-beta by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IFN-beta (500-P32B) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IFN-beta.
Western Blot: To detect Human IFN-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IFN-beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P32BBT-1MG will be provided as 2 x 500 µg
Target Information
Type I Interferons (IFN-alpha/beta) are produced primarily in response to viral infection by Natural IFN-producing cells (IPCs) as part of the host immune response. IFNs can also inhibit the development of tumors. IFN-beta binding results in the activation of the tyrosine kinases Jak1 and Tyk2, phosphorylation of members of the STAT family of transcription factors, and the transcription and expression of the immune response genes. More recently, several members of the toll-like receptor (TLR) family were found to stimulate the production IFN-beta. IFN-beta is currently used clinically for treatment of tumors, infections and multiple sclerosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Fibroblast interferon; IFN b; IFN β; IFN-beta; IFNbeta; IFNβ; Interferon; Interferon b; Interferon beta; interferon, beta 1, fibroblast; interferon-beta; RP11-113D19.1; unnamed protein product
Gene Aliases: IFB; IFF; IFN-beta; IFNB; IFNB1
UniProt ID: (Human) P01574
Entrez Gene ID: (Human) 3456
adaptive immune response
T cell activation involved in immune response
B cell activation involved in immune response
natural killer cell activation involved in immune response
negative regulation of immune system process
regulation of transcription from RNA polymerase II promoter
response to stress
defense response
immune response
humoral immune response
signal transduction
cell surface receptor signaling pathway
JAK-STAT cascade
response to virus
positive regulation of metabolic process
positive regulation of autophagy
cytokine-mediated signaling pathway
natural killer cell activation
cellular response to interferon-beta
B cell proliferation
response to exogenous dsRNA
negative regulation of viral genome replication
innate immune response
positive regulation of innate immune response
leukocyte activation
regulation of MHC class I biosynthetic process
negative regulation of T cell differentiation
positive regulation of transcription from RNA polymerase II promoter
negative regulation of multicellular organismal process
defense response to virus
type I interferon signaling pathway
neuron cellular homeostasis
cellular response to dsRNA
cellular response to exogenous dsRNA
receptor signaling pathway via STAT
cellular response to virus
negative regulation of Lewy body formation
antiviral innate immune response
positive regulation of protein localization to nucleus
negative regulation of T-helper 2 cell cytokine production
positive regulation of apoptotic signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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